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Comparison of the virucidal efficacy of peracetic acid, potassium monopersulphate and sodium hypochlorite on bacteriophages P001 and MS2
- Source :
- Journal of Applied Microbiology, Journal of Applied Microbiology, Wiley, 2015, 119 (3), pp.655-665. ⟨10.1111/jam.12870⟩
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Aims The phagicidal activity of peroxy products against the virulent bacteriophage P001 infecting lactic acid bacteria and bacteriophage MS2 used as a surrogate of enteric viruses (EVs) was evaluated and compared to sodium hypochlorite using the EN 13610 European suspension test and a surface test developed in our laboratories. Methods and results Infectivity tests were adapted and/or developed to determine the activity of disinfectants against reference P001 phage of Lactoccocus lactis and F-specific RNA phage MS2 of Escherichia coli in conditions simulating practical use. Similar concentrations of sodium hypochlorite were phagicidal against both bacteriophages, either at 0·05-0·125% of active chlorine using the suspension test or at 0·12-0·5% using the surface test. For Potassium monopersulphate (MPS), phagicidal concentrations varied from 0·006 to 0·012% whatever the type of test and phages. However, for peracetic acid products (PAP) used in suspension, concentrations 55 times higher were necessary against MS2 (0·271%) than against P001 (0·005%). With the surface test, 0·089-0·178% concentrations of PAP were effective against MS2, but these concentrations were 16-32 times greater than needed against P001. Conclusions Sodium hypochlorite and MPS had similar phagicidal activities against P001 and MS2, but PAP did not. Significance and impact of the study This is the first comparative study to investigate through suspension and surface tests the difference in resistance to peroxy compounds between a reference bacteriophage (P001) used to evaluate phagicidal concentrations in European standards and a surrogate of EVs (MS2). Results underline the importance of validation tests on pertinent surrogates of viruses or bacteriophages to adjust the concentration of disinfectants for use in the food and water industries.
- Subjects :
- Potassium Compounds
Sodium Hypochlorite
viruses
Potassium
chemistry.chemical_element
Antiviral Agents
Applied Microbiology and Biotechnology
Microbiology
Bacteriophage
03 medical and health sciences
chemistry.chemical_compound
Peracetic acid
Bacteriophage MS2
Chlorine
Bacteriophages
Peracetic Acid
Food science
ComputingMilieux_MISCELLANEOUS
Levivirus
030304 developmental biology
0303 health sciences
biology
Sulfates
030306 microbiology
General Medicine
biology.organism_classification
Lactic acid
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
chemistry
Sodium hypochlorite
Bacteria
Disinfectants
Biotechnology
Subjects
Details
- ISSN :
- 13645072 and 13652672
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Microbiology
- Accession number :
- edsair.doi.dedup.....5a620e45670241a47ebaf9c5eb0738cb
- Full Text :
- https://doi.org/10.1111/jam.12870