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High-performance liquid chromatography determination of mycophenolic acid and its glucuronide metabolite in human plasma
- Source :
- Journal of Chromatography B: Biomedical Sciences and Applications. 708:229-234
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Two HPLC-UV assays are reported here: one is a rapid assay for mycophenolic acid (MPA) and the other is a simultaneous assay for MPA and its metabolite mycophenolic acid glucuronide (MPAG). For both methods, plasma samples (500 microl) with added internal standard were acidified and extracted using C18 solid-phase extraction cartridges. Chromatographic separation was achieved on a C18 Novapak column using a mobile phase consisting of methanol-0.05% orthophosphoric acid (40:60, v/v) for the rapid MPA assay and 30:70 for the simultaneous MPA and MPAG assay. The assays were linear over the ranges 0.1 to 50.0 mg/l for MPA and 2.8 to 225.8 mg/l for MPAG. Mean absolute recovery for all analytes was >99%. These methods are suitable for therapeutic drug monitoring and pharmacokinetic studies.
- Subjects :
- Chromatography
medicine.diagnostic_test
Metabolite
Extraction (chemistry)
Glucuronates
General Chemistry
Mycophenolic Acid
High-performance liquid chromatography
Mycophenolic acid
chemistry.chemical_compound
Glucuronides
chemistry
Pharmacokinetics
Therapeutic drug monitoring
medicine
Humans
Glucuronide
Quantitative analysis (chemistry)
Chromatography, High Pressure Liquid
medicine.drug
Subjects
Details
- ISSN :
- 03784347
- Volume :
- 708
- Database :
- OpenAIRE
- Journal :
- Journal of Chromatography B: Biomedical Sciences and Applications
- Accession number :
- edsair.doi.dedup.....5a6cfa80835389c58965155ac886dda1