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PLAC1 is involved in human trophoblast syncytialization
- Source :
- Reproductive Biology. 16:218-224
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Placenta specific protein 1 (PLAC1) is thought to be important for murine and human placentation because of its abundant expression in placenta; however, the trophoblast subtypes that express PLAC1 at the fetomaternal interface and the major role of PLAC1 in placentation are still unclear. This study investigated the expression pattern of PLAC1 at the human fetomaternal interface and its involvement in trophoblast syncytialization. Localization of PLAC1 at the fetomaternal interface was studied using in situ hybridization (ISH) and immunohistochemistry (IHC) assays. Real time RT-PCR and Western Blot were employed to exhibit the expression pattern of PLAC1 during human spontaneous syncytialization of term primary cytotrophoblast cells (CTBs). Spontaneous syncytialization of a primary term CTBs model transfected with siRNA specific to PLAC1 was used to investigate the role of PLAC1 during human trophoblast syncytialization. The results showed that PLAC1 was mainly expressed in the human villous syncytiotrophoblast (STB) layer throughout gestation, and the expression level of PLAC1 was significantly elevated during human trophoblast syncytialization. Down-regulation of PLAC1 via specific PLAC1 siRNA transfection attenuated spontaneous syncytialization of primary term CTBs (p
- Subjects :
- 0301 basic medicine
Placenta
CTBS
Down-Regulation
In situ hybridization
Pregnancy Proteins
Biology
03 medical and health sciences
Endocrinology
Syncytiotrophoblast
Pregnancy
medicine
Humans
reproductive and urinary physiology
Cytotrophoblast
Cell fusion
Gene Expression Regulation, Developmental
Trophoblast
Placentation
Trophoblasts
Cell biology
030104 developmental biology
medicine.anatomical_structure
embryonic structures
Immunology
Female
Animal Science and Zoology
Developmental Biology
Subjects
Details
- ISSN :
- 1642431X
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Reproductive Biology
- Accession number :
- edsair.doi.dedup.....5a9b2510aa04b388ee006daf21649fd2