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Growth rate regulation of lac operon expression in Escherichia coli is cyclic AMP dependent
- Source :
- FEBS Letters. (3):397-402
- Publisher :
- Published by Elsevier B.V.
-
Abstract
- In contrast to the ribosomal RNA gene expression increasing with growth rate, transcription of the lac operon is downregulated by cell growth rate. In continuous culture, growth rate regulation of lac promoter was independent of carbon substrate used and its location on the chromosome. Since the lac operon is activated by cyclic adenosine monophosphate (cAMP), which decreases with increasing cell growth rate, expression of plac-lacZ reporter fusion was analyzed in cya mutant under various growth conditions. The results demonstrated that expression of plac-lacZ in cya mutant was both lower and growth rate independent. In addition, ppGpp (guanosine tetraphosphate) was not involved in the mechanism of growth rate regulation of the lac promoter. Thus, the results of this study indicate that cAMP mediates the growth rate-dependent regulation of lac operon expression in Escherichia coli.
- Subjects :
- Cyclic AMP Receptor Protein
Genotype
Transcription, Genetic
Mutant
Biophysics
lac operon
Guanosine Tetraphosphate
Lac repressor
Biology
medicine.disease_cause
Biochemistry
trp operon
Cyclic adenosine monophosphate
chemistry.chemical_compound
Species Specificity
Structural Biology
Transcription (biology)
Genetics
medicine
Cyclic AMP
Escherichia coli
Bacteriophages
Molecular Biology
Growth rate
Cell Biology
beta-Galactosidase
Molecular biology
Carbon
Phenotype
chemistry
Gene Expression Regulation
Mutation
bacteria
Gene Deletion
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 00145793
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....5ae404ec1f8003b58f73ef663ce1b47f
- Full Text :
- https://doi.org/10.1016/S0014-5793(03)01071-8