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Aspartic acid incorporated monolithic columns for affinity glycoprotein purification
- Publication Year :
- 2014
- Publisher :
- Elsevier Science BV, 2014.
-
Abstract
- WOS: 000331596100009<br />PubMed ID: 24161508<br />Novel aspartic acid incorporated monolithic columns were prepared to efficiently affinity purify immunoglobulin G (IgG) from human plasma. The monolithic columns were synthesised in a stainless steel HPLC column (20 cm x 5 mm id) by in situ bulk polymerisation of N-methacryloyl-L-aspartic acid (MAAsp), a polymerisable derivative of L-aspartic acid, and 2-hydroxyethyl methacrylate (HEMA). Monolithic columns [poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-aspartic acid) (PHEMAsp)] were characterised by swelling studies, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The monolithic columns were used for IgG adsorption/desorption from aqueous solutions and human plasma. The IgG adsorption depended on the buffer type, and the maximum IgG adsorption from aqueous solution in phosphate buffer was 0.085 mg/g at pH 6.0. The monolithic columns allowed for one-step IgG purification with a negligible capacity decrease after ten adsorption-desorption cycles
- Subjects :
- Methacrylate
Ligands
High-performance liquid chromatography
Chromatography, Affinity
Colloid and Surface Chemistry
Adsorption
Desorption
Aspartic acid
Humans
Physical and Theoretical Chemistry
Fourier transform infrared spectroscopy
Serum Albumin
Glycoproteins
Aspartic Acid
Aqueous solution
Chromatography
Chemistry
Pseudospecific Ligands
Osmolar Concentration
Temperature
Surfaces and Interfaces
General Medicine
Hydrogen-Ion Concentration
Monolithic Columns
High Performance Liquid Chromatography
Solutions
Polymerization
Immunoglobulin G
Microscopy, Electron, Scanning
Methacrylates
Igg Separation
Rheology
Biotechnology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....5b0823c08f8a70a9ee72f347c53e6bca