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Cancer stem cell-related gene periostin: a novel prognostic marker for breast cancer
- Source :
- PLoS ONE, Vol 7, Iss 10, p e46670 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- We investigated the expression status of periostin in breast cancer stem cells and its clinical implications in order to lay a foundation for managing breast cancer. CD44+/CD24−/line- tumor cells (CSC) from clinical specimens were sorted using flow cytometry. Periostin expression status was detected in CSC cells and 1,086 breast cancer specimens by Western blot and immunohistochemistry staining, with the CSC ratio determined by immunofluorescence double staining. The relationship between the periostin protein and clinico-pathological parameters and prognosis was subsequently determined. As a result, CSC cells are more likely to generate new tumors in mice and cell microspheres that are deficient in NOD/SCID compared to the control group. Periostin protein was expressed higher in CSC cells compared to the control cells and was found to be related to CSC chemotherapy resistance. Moreover, periostin expression was found to be related to the CSC ratio in 1,086 breast cancer specimens (P = 0.001). In total, 334 (30.76%) of the 1,086 breast cases showed high periostin expression. After universal and Spearman regression correlation analysis, periostin was observed to be related to histological grade, CSC ratio, lymph node metastasis, tumor size, and triple-negative breast cancer (all P
- Subjects :
- Cytoplasm
Pathology
Cancer Treatment
lcsh:Medicine
Metastasis
Tumor Cells, Cultured
lcsh:Science
Aged, 80 and over
Multidisciplinary
Obstetrics and Gynecology
Bone metastasis
Gene Therapy
Middle Aged
Immunohistochemistry
Oncology
Neoplastic Stem Cells
Medicine
Female
Stem cell
Cancer Prevention
Research Article
Adult
medicine.medical_specialty
Cell Survival
Blotting, Western
Breast Neoplasms
In Vitro Techniques
Periostin
Biology
Breast cancer
Diagnostic Medicine
Cancer stem cell
Breast Cancer
Cancer Detection and Diagnosis
Early Detection
Biomarkers, Tumor
medicine
Humans
Aged
Cell Membrane
CD44
lcsh:R
Cancers and Neoplasms
Chemotherapy and Drug Treatment
medicine.disease
General Surgery
biology.protein
Cancer research
Surgery
lcsh:Q
Cell Adhesion Molecules
Biomarkers
General Pathology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....5b1dbb77405f3e09a72a13c8da6f60ea