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BoHV-4 immediate early 1 gene is a dispensable gene and its product is not a bone marrow stromal cell antigen 2 counteracting factor
- Source :
- BMC Veterinary Research
- Publisher :
- Springer Nature
-
Abstract
- Background Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus whose genome was cloned as Bacterial Artificial Chromosome (BAC) and exploited as a gene delivery vector for vaccine purposes. Although BoHV-4 genome has been completely sequenced and its open reading frames (ORFs) structurally defined in silico, most of them are not functionally characterized. In BoHV-4 genome two major immediate early genes (IE) are present, IE1 and IE2. IE2 is an essential gene because its removal from the viral genome renders the virus unable to replicate, whereas for IE1 no many functional information are available. Results In this work, IE1 contribution in initiating and maintaining BoHV-4 lytic replication was assessed generating a recombinant BoHV-4 genome lacking of IE1 gene, BoHV-4ΔIE1. In contrast to BoHV-4IE2 deleted mutant, BoHV-4ΔIE1 infectious replicating viral particles (IRVPs) could be reconstituted following viral DNA electroporation in permissive cells. However the titer of BoHV-4ΔIE1 IRVPs produced into the cell supernatant and BoHV-4ΔIE1 plaques size were reduced respect to BoHV-4 undeleted control. Further the impaired BoHV-4ΔIE1 IRVPs produced into the cell supernatant could be rescued by expressing IE1 gene product in trans, confirming the implication of IE1 in BoHV-4 lytic replication. Next, the possible role of BoHV-4IE1 as bone marrow stromal cell antigen 2 (BST-2) counteracting factor, as hypothesized by IE1 amino-terminal gene product homology with Kaposi Sarcoma Associated Herpesvirus (KSHV) K5, was excluded too. Conclusions Although the real function of BoHV-4IE1 is still elusive, a new BoHV-4 genome gene locus as a target site for the insertion of foreign DNA and resulting in the attenuation of the virus has been revealed. These data can be considered of relevance to improve BoHV-4 gene delivery properties. Electronic supplementary material The online version of this article (doi:10.1186/s12917-015-0540-4) contains supplementary material, which is available to authorized users.
- Subjects :
- Gene Expression Regulation, Viral
Chromosomes, Artificial, Bacterial
Genome evolution
viruses
Bacteria artificial chromosome
Genome, Viral
Viral Plaque Assay
Biology
Gene delivery
Virus Replication
Gene dosage
Cell Line
Viral vector
Immediate early gene
Gene product
Gene knock-down
Animals
Humans
Bovine herpesvirus 4
Genes, Immediate-Early
Gene
Regulation of gene expression
General Veterinary
virus diseases
Mesenchymal Stem Cells
General Medicine
Virology
veterinary(all)
Herpesvirus 4, Bovine
Viral replication
Impaired viral replication
Gene Deletion
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 17466148
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Veterinary Research
- Accession number :
- edsair.doi.dedup.....5b28ee4bdbbdadd7326e9874722107e2
- Full Text :
- https://doi.org/10.1186/s12917-015-0540-4