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RING finger palmitoylation of the endoplasmic reticulum Gp78 E3 ubiquitin ligase
- Source :
- FEBS Letters. 586:2488-2493
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- Gp78 is an E3 ubiquitin ligase within the endoplasmic reticulum-associated degradation pathway. We show that Flag-tagged gp78 undergoes sulfhydryl cysteine palmitoylation (S-palmitoylation) within the RING finger motif, responsible for its ubiquitin ligase activity. Screening of 19 palmitoyl acyl transferases (PATs) identified five that increased gp78 RING finger palmitoylation. Endoplasmic reticulum (ER)-localized Myc-DHHC6 overexpression promoted the peripheral ER distribution of Flag-gp78 while RING finger mutation and the palmitoylation inhibitor 2-bromopalmitate restricted gp78 to the central ER. Palmitoylation of RING finger cysteines therefore regulates gp78 distribution to the peripheral ER.
- Subjects :
- Gp78
Proteasome Endopeptidase Complex
Amino Acid Motifs
Palmitates
Biophysics
Palmitic Acids
Endoplasmic-reticulum-associated protein degradation
Endoplasmic Reticulum
Biochemistry
Mice
03 medical and health sciences
0302 clinical medicine
Palmitoylation
Structural Biology
Chlorocebus aethiops
Genetics
Ring finger
medicine
Animals
Molecular Biology
Degradation pathway
030304 developmental biology
0303 health sciences
Trafficking
Binding Sites
biology
Chemistry
Endoplasmic reticulum
technology, industry, and agriculture
Cell Biology
ERAD
Protein Structure, Tertiary
Cell biology
Ubiquitin ligase
Receptors, Autocrine Motility Factor
Protein Transport
S-palmitoylation
medicine.anatomical_structure
E3 ubiquitin ligase
Gene Expression Regulation
Microscopy, Fluorescence
COS Cells
Mutation
Peripheral ER
biology.protein
lipids (amino acids, peptides, and proteins)
030217 neurology & neurosurgery
Cysteine
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 586
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....5b5a1082fc83b01dbbcdc734b6b8c69e