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Multiphoton imaging of tumor biomarkers with conjugates of single-domain antibodies and quantum dots

Multiphoton imaging of tumor biomarkers with conjugates of single-domain antibodies and quantum dots

Authors :
Hilal Hafian
Jean-Marc Millot
Michel Pluot
Patrick Chames
Daniel Baty
Jacques H. M. Cohen
Marc Turini
Alyona Sukhanova
Igor Nabiev
Laboratoire de Recherche en Nanosciences - EA 4682 (LRN)
Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé)
Université de Reims Champagne-Ardenne (URCA)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet
Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)
Aix Marseille Université (AMU)
SFR Condorcet
Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé)
Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)
Source :
Nanomedicine: Nanotechnology, Biology and Medicine, Nanomedicine: Nanotechnology, Biology and Medicine, 2014, 10 (8), pp.1701-1709. ⟨10.1016/j.nano.2014.05.014⟩, Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2014, 10 (8), pp.1701-1709. ⟨10.1016/j.nano.2014.05.014⟩
Publication Year :
2014
Publisher :
HAL CCSD, 2014.

Abstract

An ideal multiphoton fluorescent nanoprobe should combine a nanocrystal with the largest possible two-photon absorption cross section (TPACS) and the smallest highly specific recognition molecules bound in an oriented manner. CdSe/ZnS quantum dots (QDs) conjugated to 13-kDa single-domain antibodies (sdAbs) derived from camelid IgG or streptavidin have been used as efficient two-photon excitation (TPE) probes for carcinoembryonic antigen (CEA) imaging on normal human appendix and colon carcinoma tissue. The TPACS for some conjugates was higher than 49,000 GM (Goeppert-Mayer units), considerably exceeding that of organic dyes being close to the theoretical value of 50,000 GM calculated for CdSe QDs. The ratio of sdAb-QD emission to the autofluorescence for 800 nm TPE was 40 times higher than that for 457.9 nm one-photon excitation. TPE ensures a clear discrimination of CEA-overexpressing tumor areas from normal tissue. Oriented sdAb-QD conjugates are bright specific labels for detecting low concentrations of antigens using multiphoton microscopy. From the Clinical Editor This study demonstrates carcinoembryonic antigen imaging on normal human appendix and colon carcinoma tissue utilizing CdSe/ZnS quantum dots conjugated to streptavidin or to 13-kDa single-domain antibodies as efficient two-photon excitation probes.

Details

Language :
English
ISSN :
15499634
Database :
OpenAIRE
Journal :
Nanomedicine: Nanotechnology, Biology and Medicine, Nanomedicine: Nanotechnology, Biology and Medicine, 2014, 10 (8), pp.1701-1709. ⟨10.1016/j.nano.2014.05.014⟩, Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2014, 10 (8), pp.1701-1709. ⟨10.1016/j.nano.2014.05.014⟩
Accession number :
edsair.doi.dedup.....5b65be368177e89218278e96f5f99f03
Full Text :
https://doi.org/10.1016/j.nano.2014.05.014⟩