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Separation of lignocresol from eucalyptus lignocresol-cellulase complex using organic solvents
- Source :
- Bioresource technology. 143
- Publication Year :
- 2013
-
Abstract
- A functional lignin-based material, lignocresol, was synthesized from eucalyptus wood meal by using phase separation treatment with p-cresol and 72% sulfuric acid. Lignocresol physically adsorbs Trichoderma reesei cellulase and functions as an immobilized cellulase. Lignocresol-immobilized cellulase was treated with acetone or ethanol to separate the enzyme and its support. Most lignocresol was dissolved in organic solvents and then precipitated in diethyl ether. The recovery yield of lignocresol using acetone was 85% and that using ethanol was 72%, with very low or negligible residual cellulase. Lignocresol is an innovative recyclable enzyme support that can be easily separated using only organic solvents after deactivation of the immobilized enzyme.
- Subjects :
- Environmental Engineering
Immobilized enzyme
Bioengineering
Cellulase
Lignin
chemistry.chemical_compound
Cresols
Acetone
Organic chemistry
Organic Chemicals
Waste Management and Disposal
Trichoderma reesei
Eucalyptus
Ethanol
biology
Renewable Energy, Sustainability and the Environment
Sulfuric acid
General Medicine
biology.organism_classification
Enzymes, Immobilized
chemistry
biology.protein
Solvents
Diethyl ether
Subjects
Details
- ISSN :
- 18732976
- Volume :
- 143
- Database :
- OpenAIRE
- Journal :
- Bioresource technology
- Accession number :
- edsair.doi.dedup.....5b9c382af41215df59cab75c70a7124c