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Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63‐mediated transcription of KRT10 to improve psoriasis
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Psoriasis is a chronic immune‐mediated inflammatory dermatosis. Recently, ozone therapy has been applicated to psoriasis treatment; however, the mechanism by which ozone therapy improves psoriasis remains unclear. The excessive proliferation and the differentiation of basal keratinocytes have been considered critical issues during pathological psoriasis process, in which keratin 6 (KRT6) and KRT10 might be involved. In the present study, KRT6, IL‐17 and IL‐22 protein within psoriasis lesions was decreased, while KRT10 and Tp63 protein in psoriasis lesions was increased by ozone treatment in both patient and IMQ mice psoriatic tissues. In the meantime, ozone treatment down‐regulated KRT6 mRNA and protein expression while up‐regulated KRT10 mRNA and protein expression within IL‐22 treated primary KCs; the cell viability of KCs was suppressed by ozone treatment. Moreover, Tp63 bound to KRT10 promoter region to activate its transcription in basal keratinocytes; the promotive effects of ozone on Tp63 and KRT10 were significantly reversed by Tp63 silence. Both TP63 and KRT10 mRNA expression were significantly increased by ozone treatment in psoriasis lesions; there was a positive correlation between Tp63 and KRT10 expression within tissue samples, suggesting that ozone induces the expression of Tp63 to enhance the expression of KRT10 and the differentiation of keratinocytes, therefore improving the psoriasis. In conclusion, the application of ozonated oil could be an efficient and safe treatment for psoriasis; ozone promotes the differentiation of keratinocytes via increasing Tp63‐mediated transcription of KRT10, therefore improving psoriasis.
- Subjects :
- Adult
Keratinocytes
Male
0301 basic medicine
Primary Cell Culture
Apoptosis
Dermatitis
keratin 6 (KRT6)/KRT10
Mice
03 medical and health sciences
Ozone
0302 clinical medicine
Transcription (biology)
Psoriasis
TP63
Keratin
medicine
Animals
Humans
Viability assay
Tp63
Cell Proliferation
Skin
ozone therapy
chemistry.chemical_classification
Messenger RNA
business.industry
Tumor Suppressor Proteins
Keratin-6
Cell Differentiation
Promoter
Original Articles
Cell Biology
Keratin-10
Ozone therapy
medicine.disease
Disease Models, Animal
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Female
Original Article
business
Transcription Factors
keratinocytes (KCs)
Subjects
Details
- ISSN :
- 15824934 and 15821838
- Volume :
- 24
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....5b9df497e1eac95e5f939f47b1bf388e