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Characterization and Whole Genome Sequencing of AR23, a Highly Toxic Bacillus thuringiensis Strain Isolated from Lebanese Soil
- Source :
- Current Microbiology, Current Microbiology, Springer Verlag, 2019, 76 (12), pp.1503-1511. ⟨10.1007/s00284-019-01775-9⟩, Current Microbiology, Vol. 76, no.12, p. 1503-1511 (2019)
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- The demand for sustainable and eco-friendly control methods of pests and insects is increasing worldwide. From this came the interest in Bacillus thuringiensis, an entomopathogenic bacterium capable of replacing chemical pesticides. However, the possibility of pests developing resistance to a particular strain may impair its use, and there is a need to identify novel strains of this species as potential commercial biopesticides. B. thuringiensis sv. israelensis is one of the most successful serovars, widely commercialized for its activity against black fly and mosquito larvae. In this study, we isolated, characterized, and sequenced a new Lebanese B. thuringiensis sv. israelensis isolate, strain AR23. Compared to the commercialized reference strain AM65-52 (Vectobac®, Sumitomo), AR23 showed an increased activity against several mosquito species. The genomic analysis revealed that this strain, compared to AM65-52, possesses a simplified plasmid content and an additional functional cry4Ba coding gene that most likely accounts for the increased effectiveness of this strain in mosquito larvae killing.
- Subjects :
- [SDV]Life Sciences [q-bio]
Bacillus thuringiensis
PROTEIN
Mosquito Vectors
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Hemolysin Proteins
Plasmid
Bacterial Proteins
Animals
[CHIM]Chemical Sciences
Lebanon
Phylogeny
Soil Microbiology
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Whole genome sequencing
0303 health sciences
DELTA-ENDOTOXIN
CRYSTAL
biology
Strain (chemistry)
Bacillus thuringiensis Toxins
030306 microbiology
COMPONENTS
fungi
General Medicine
Pesticide
biology.organism_classification
GENE
MOSQUITO
Endotoxins
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics
Biopesticide
Larva
SUBSP ISRAELENSIS
INFERENCE
RESISTANCE
Bacteria
Genome, Bacterial
GENERATION
Delta endotoxin
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 03438651 and 14320991
- Database :
- OpenAIRE
- Journal :
- Current Microbiology, Current Microbiology, Springer Verlag, 2019, 76 (12), pp.1503-1511. ⟨10.1007/s00284-019-01775-9⟩, Current Microbiology, Vol. 76, no.12, p. 1503-1511 (2019)
- Accession number :
- edsair.doi.dedup.....5bad23ae59dd116c030a2a83ef9076f3