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3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation
- Source :
- Journal of Tissue Engineering, Vol 10 (2019), Journal of Tissue Engineering
- Publication Year :
- 2019
-
Abstract
- Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.
- Subjects :
- food.ingredient
human platelet lysate
Biomedical Engineering
Medicine (miscellaneous)
Adipose tissue
macromolecular substances
02 engineering and technology
Gelatin
lcsh:Biochemistry
Biomaterials
human mesenchymal stem cells
03 medical and health sciences
food
bone regeneration
Tissue engineering
human mesenchymal stem cell
medicine
lcsh:QD415-436
Mesenchymal stem cell proliferation
Bone regeneration
030304 developmental biology
0303 health sciences
Hybrid chitosan-gelatin hydrogel
tissue engineering
Chemistry
Mesenchymal stem cell
technology, industry, and agriculture
021001 nanoscience & nanotechnology
3. Good health
Cell biology
carbohydrates (lipids)
medicine.anatomical_structure
Self-healing hydrogels
Original Article
Bone marrow
0210 nano-technology
Subjects
Details
- ISSN :
- 20417314
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of tissue engineering
- Accession number :
- edsair.doi.dedup.....5bbfe4d3ee2e4512ba65adc42b431d5d