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3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation

Authors :
Domenico Russo
Luigi Fabrizio Rodella
Fabio Savoldi
Cristina Manferdini
Gina Lisignoli
Kamol Dey
Luciana Sartore
Manuel Salmerón-Sánchez
Silvia Agnelli
Simona Bernardi
Vladimira Moulisova
Corrado Paganelli
Andrea Bianchetti
Fulvio Magni
Nicola Lopomo
Emilio Sardini
Federica Re
Camillo Almici
Marco Cantini
Elisa Borsani
Clizia Chinello
Pierangelo Guizzi
Re, F
Sartore, L
Moulisova, V
Cantini, M
Almici, C
Bianchetti, A
Chinello, C
Dey, K
Agnelli, S
Manferdini, C
Bernardi, S
Lopomo, N
Sardini, E
Borsani, E
Rodella, L
Savoldi, F
Paganelli, C
Guizzi, P
Lisignoli, G
Magni, F
Salmeron-Sanchez, M
Russo, D
Source :
Journal of Tissue Engineering, Vol 10 (2019), Journal of Tissue Engineering
Publication Year :
2019

Abstract

Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.

Details

ISSN :
20417314
Volume :
10
Database :
OpenAIRE
Journal :
Journal of tissue engineering
Accession number :
edsair.doi.dedup.....5bbfe4d3ee2e4512ba65adc42b431d5d