Perfomance of different embryo stage of sweet orange x mandarin cultivated in vitro

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:47:31Z No. of bitstreams: 1 S0100-29452003000300040.pdf: 357439 bytes, checksum: bf5210c5b42fd18d4bf8582a25c0255d (MD5) Made available in DSpace on 2013-08-22T18:47:31Z (GMT). No. of bitstreams: 1 S0100-29452003000300040.pdf: 357439 bytes, checksum: bf5210c5b42fd18d4bf8582a25c0255d (MD5) Previous issue date: 2003-12-01 Made available in DSpace on 2013-09-30T18:00:48Z (GMT). No. of bitstreams: 2 S0100-29452003000300040.pdf: 357439 bytes, checksum: bf5210c5b42fd18d4bf8582a25c0255d (MD5) S0100-29452003000300040.pdf.txt: 13904 bytes, checksum: 3e42e7a1b86ce6a695f5874fa7ff394a (MD5) Previous issue date: 2003-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:17:51Z No. of bitstreams: 2 S0100-29452003000300040.pdf: 357439 bytes, checksum: bf5210c5b42fd18d4bf8582a25c0255d (MD5) S0100-29452003000300040.pdf.txt: 13904 bytes, checksum: 3e42e7a1b86ce6a695f5874fa7ff394a (MD5) Made available in DSpace on 2014-05-20T13:17:51Z (GMT). No. of bitstreams: 2 S0100-29452003000300040.pdf: 357439 bytes, checksum: bf5210c5b42fd18d4bf8582a25c0255d (MD5) S0100-29452003000300040.pdf.txt: 13904 bytes, checksum: 3e42e7a1b86ce6a695f5874fa7ff394a (MD5) Previous issue date: 2003-12-01 Objetivou-se verificar qual o melhor estádio embrionário para o cultivo de embriões imaturos oriundos de frutos provenientes de hibridação entre 'Pêra Rio' x 'Poncã' , bem como o efeito de diferentes concentrações do meio de cultura MT. Os embriões em diferentes estádios de desenvolvimento (globulares, torpedo e cordiforme) foram excisados e inoculados em tubos de ensaio contendo 15 mL do meio MT com diferentes concentrações (0; 50; 100 e 150% da composição original e acrescido de 50 g.L-1 de sacarose). Após a inoculação, os embriões foram incubados à 27±1ºC, fotoperíodo de 16 horas e irradiância de 32 mmol.m-2.s-1. Após 90 dias, avaliou-se o comprimento da parte aérea e do sistema radicular, massa fresca e número de folhas das plântulas. Melhor desenvolvimento dos embriões imaturos foi obtido em estádio cotiledonar e com a concentração de 150% do meio MT. Embryos obtained from Citrus sinensis Osbeck 'Pêra Rio' x Citrus reticulata Blanco 'Poncã' crossings were excised in diverse phases of development and cultivated in different medium concentrations. Embryos in globular, torped and cotiledonary stage were excised and inoculated individually in test tubes with 15 mL in distinct MT medium concentrations (0; 50; 100 and 150% of original composition and added sucrose (50 g.L-1). After inoculation, the embryos were maintained in growth room at 27±1ºC, 16 hour photoperiod and 32 umol.m-2.s-1 irradiance. After 90 days, the length of the aerial part and roots system, fresh mass and number of leaves, were evaluated. The best results were obtained by incubating embryos in the cotiledonary stage, in MT medium with concentrations of 150%. UNESP FCAV Departamento de Tecnologia Universidade Federal de Lavras (UFLA) Departamento de Agricultura FAPEMIG UNESP FCAV Departamento de Tecnologia