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Positron-Emission Tomography Imaging of Early Events after Transplantation of Islets of Langerhans
- Source :
- Transplantation, Vol. 79, No 3 (2005) pp. 353-5
- Publication Year :
- 2005
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2005.
-
Abstract
- The aim of our study was to assess cell trafficking and early events after intraportal islet transplantation. Sprague-Dawley rat islets were incubated for various times, with various concentrations of 2-[F]fluoro-2deoxy-D-glucose (FDG), and in presence of various glucose concentrations. FDG-labeled syngeneic islets or FDG alone were injected in rats. Radioactivity was measured in the liver and in various organs by positron-emission tomography for 6 hours. FDG uptake increased with incubation time or FDG concentration and decreased in presence of glucose. In vivo, all islets implanted in the liver, with an uptake 4.4 times higher than controls (44.2% vs. 10.1%, P=0.02). Radioactivity in the liver decreased at the same rate after injection of labeled-islets and FDG alone. Ex vivo labeling of islets and imaging of posttransplant early events were feasible. Islets engrafted exclusively in the liver. No islet loss could be demonstrated 6 hours after transplantation.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
endocrine system diseases
Cell
Islets of Langerhans Transplantation
ddc:616.0757
Incubation period
Rats, Sprague-Dawley
Islets of Langerhans
Fluorodeoxyglucose F18
In vivo
Internal medicine
medicine
Animals
Tissue Distribution
Transplantation
geography
geography.geographical_feature_category
ddc:617
medicine.diagnostic_test
business.industry
Fdg uptake
Fluorodeoxyglucose F18/diagnostic use/pharmacokinetics
Islet
Rats
Islets of Langerhans Transplantation/physiology
Radiopharmaceuticals/diagnostic use/pharmacokinetics
Endocrinology
medicine.anatomical_structure
Positron emission tomography
Positron-Emission Tomography
Islets of Langerhans/radionuclide imaging
Radiopharmaceuticals
business
Ex vivo
Subjects
Details
- ISSN :
- 00411337
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Transplantation
- Accession number :
- edsair.doi.dedup.....5bc836c35279f66eb8dc5605e5281f33