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Screening and Identification of a Phage Display Derived Peptide That Specifically Binds to the CD44 Protein Region Encoded by Variable Exons
- Source :
- SLAS Discovery. 21:44-53
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- CD44, especially the isoforms with variable exons (CD44v), is a promising biomarker for the detection of cancer. To develop a CD44v-specific probe, we screened a 7-mer phage peptide library against the CD44v3-v10 protein using an improved subtractive method. The consensus sequences with the highest frequency (designated CV-1) emerged after four rounds of panning. The binding affinity and specificity of the CV-1 phage and the synthesized peptide for the region of CD44 encoded by the variable exons were confirmed using enzyme-linked immunosorbent assay and competitive inhibition assays. Furthermore, the binding of the CV-1 probe to gastric cancer cells and tissues was validated using immunofluorescence and immunohistochemistry assays. CV-1 sensitively and specifically bound to CD44v on cancer cells and tissues. Thus, CV-1 has the potential to serve as a promising probe for cancer molecular imaging and target therapy.
- Subjects :
- 0301 basic medicine
Phage display
Fluorescent Antibody Technique
Peptide
Plasma protein binding
Immunofluorescence
Binding, Competitive
Sensitivity and Specificity
Biochemistry
Cell Line
Analytical Chemistry
03 medical and health sciences
Peptide Library
Stomach Neoplasms
Cell Line, Tumor
Consensus sequence
medicine
Humans
Bacteriophages
Panning (camera)
Peptide library
chemistry.chemical_classification
biology
medicine.diagnostic_test
CD44
Exons
Immunohistochemistry
Molecular biology
HEK293 Cells
Hyaluronan Receptors
030104 developmental biology
chemistry
biology.protein
Molecular Medicine
Peptides
Protein Binding
Biotechnology
Subjects
Details
- ISSN :
- 24725552
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- SLAS Discovery
- Accession number :
- edsair.doi.dedup.....5bf303cda9f0b9ae6fce2ab3118ade75