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Oral administration of Clostridium butyricum rescues streptomycin-exacerbated respiratory syncytial virus-induced lung inflammation in mice
- Source :
- Virulence, Vol 12, Iss 1, Pp 2133-2148 (2021), Virulence, article-version (VoR) Version of Record
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis Group, 2021.
-
Abstract
- Changes in the intestinal microbiota indirectly impact the health of mucosa distal to the intestine, particularly the respiratory tract. However, the effects of intestinal microbiota dysbiosis on the regulation of respiratory syncytial virus (RSV) infection are not clear. In this study, we examined the effects of altering the intestinal microbiota on the pulmonary immune response against RSV infection. BALB/c mice were treated with streptomycin before infection with RSV to study the altered immune response. The ingestion of streptomycin led to a marked alteration in the intestinal microbiota with a reduced abundance of Lactobacillus and Clostridium genera, followed by greatly aggravated pulmonary inflammation in response to RSV infection. This aggravated inflammation was associated with a dysregulated immune response against RSV infection, characterized by the increased expression of IFN-γ and IL-17 and increased pulmonary M1-like macrophage polarization, and decreased expression of IL-5. Supplementation of Clostridium butyricum (CB) prevented aggravated inflammation and the dysregulated immune response characterized by greater M2 polarization of pulmonary macrophages and decreased release of IFN-γ and IL-17 as well as increased IL-5 levels. Furthermore, in vitro and in vivo experiments identified that butyrate, the main metabolite produced by CB, promoted M2 polarization of macrophages in RSV-infected mice exposed to streptomycin. Together, these results demonstrate the mechanism by which intestinal microbiota modulate the pulmonary immune response to RSV infection.
- Subjects :
- Microbiology (medical)
Immunology
Pneumonia, Viral
Macrophage polarization
Administration, Oral
Inflammation
rsv
Respiratory Syncytial Virus Infections
Infectious and parasitic diseases
RC109-216
Microbiology
immune response
Interferon-gamma
Mice
Immune system
Lactobacillus
medicine
Animals
Clostridium butyricum
Mice, Inbred BALB C
Lung
gastrointestinal microbiota
biology
Interleukin-17
biology.organism_classification
medicine.disease
butyrate
Gastrointestinal Microbiome
macrophages
Infectious Diseases
medicine.anatomical_structure
Respiratory Syncytial Virus, Human
clostridium butyricum
Streptomycin
Dysbiosis
Parasitology
medicine.symptom
Interleukin-5
Respiratory tract
Research Article
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 21505608 and 21505594
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virulence
- Accession number :
- edsair.doi.dedup.....5c2fe7a19fd4f28eb3f6a052509ff464