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Hexadecenoic fatty acid positional isomers and De Novo PUFA synthesis in colon cancer cells

Authors :
Anna Sansone
Marco De Spirito
Roberta Scanferlato
Carla Ferreri
Letizia Polito
Massimo Bortolotti
Andrea Bolognesi
Chryssostomos Chatgilialoglu
Giuseppe Maulucci
Scanferlato, Roberta
Bortolotti, Massimo
Sansone, Anna
Chatgilialoglu, Chryssostomo
Polito, Letizia
De Spirito, Marco
Maulucci, Giuseppe
Bolognesi, Andrea
Ferreri, Carla
Source :
International journal of molecular sciences, 20 (2019): 832. doi:10.3390/ijms20040832, info:cnr-pdr/source/autori:Scanferlato R.; Bortolotti M.; Sansone A.; Chatgilialoglu C.; Polito L.; de Spirito M.; Maulucci G.; Bolognesi A.; Ferreri C./titolo:Hexadecenoic fatty acid positional isomers and De Novo PUFA synthesis in colon cancer cells/doi:10.3390%2Fijms20040832/rivista:International journal of molecular sciences (Print)/anno:2019/pagina_da:832/pagina_a:/intervallo_pagine:832/volume:20, International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 20, Iss 4, p 832 (2019), Volume 20, Issue 4
Publication Year :
2019
Publisher :
Molecular Diversity Preservation International MDPI, Basel (Matthaeustrasse 11), 2019.

Abstract

Palmitic acid metabolism involves delta-9 and delta-6 desaturase enzymes forming palmitoleic acid (9cis-16:1<br />n-7 series) and sapienic acid (6cis-16:1<br />n-10 series), respectively. The corresponding biological consequences and lipidomic research on these positional monounsaturated fatty acid (MUFA) isomers are under development. Furthermore, sapienic acid can bring to the de novo synthesis of the n-10 polyunsaturated fatty acid (PUFA) sebaleic acid (5cis,8cis-18:2), but such transformations in cancer cells are not known. The model of Caco-2 cell line was used to monitor sapienic acid supplementation (150 and 300 &mu<br />M) and provide evidence of the formation of n-10 fatty acids as well as their incorporation at levels of membrane phospholipids and triglycerides. Comparison with palmitoleic and palmitic acids evidenced that lipid remodelling was influenced by the type of fatty acid and positional isomer, with an increase of 8cis-18:1, n-10 PUFA and a decrease of saturated fats in case of sapienic acid. Cholesteryl esters were formed only in cases with sapienic acid. Sapienic acid was the less toxic among the tested fatty acids, showing the highest EC50s and inducing death only in 75% of cells at the highest concentration tested. Two-photon fluorescent microscopy with Laurdan as a fluorescent dye provided information on membrane fluidity, highlighting that sapienic acid increases the distribution of fluid regions, probably connected with the formation of 8cis-18:1 and the n-10 PUFA in cell lipidome. Our results bring evidence for MUFA positional isomers and de novo PUFA synthesis for developing lipidomic analysis and cancer research.

Details

Language :
English
Database :
OpenAIRE
Journal :
International journal of molecular sciences, 20 (2019): 832. doi:10.3390/ijms20040832, info:cnr-pdr/source/autori:Scanferlato R.; Bortolotti M.; Sansone A.; Chatgilialoglu C.; Polito L.; de Spirito M.; Maulucci G.; Bolognesi A.; Ferreri C./titolo:Hexadecenoic fatty acid positional isomers and De Novo PUFA synthesis in colon cancer cells/doi:10.3390%2Fijms20040832/rivista:International journal of molecular sciences (Print)/anno:2019/pagina_da:832/pagina_a:/intervallo_pagine:832/volume:20, International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 20, Iss 4, p 832 (2019), Volume 20, Issue 4
Accession number :
edsair.doi.dedup.....5c388f16430be2502e9b7196c8b01e77
Full Text :
https://doi.org/10.3390/ijms20040832