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Photodynamic Inactivation of an Endodontic Bacteria Using Diode Laser and Indocyanine Green-Loaded Nanosphere
- Source :
- International Journal of Molecular Sciences, Volume 22, Issue 16, International Journal of Molecular Sciences, Vol 22, Iss 8384, p 8384 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Apical periodontitis, an inflammatory lesion causing bone resorption around the apex of teeth, is treated by eradicating infectious bacteria from the root canal. However, it has a high recurrence rate and often requires retreatment. We investigated the bactericidal effect of antimicrobial photodynamic therapy (aPDT)/photodynamic antimicrobial chemotherapy (PACT) using indocyanine green (ICG)-loaded nanospheres coated with chitosan and a diode laser on a biofilm of Enterococcus faecalis, a pathogen of refractory apical periodontitis. Biofilm of E. faecalis was cultured in a porcine infected root canal model. ICG solution was injected into the root canal, which was then irradiated with a laser (810 nm wavelength) from outside the root canal. The bactericidal effect was evaluated by colony counts and scanning electron microscopy. The result of the colony counts showed a maximum 1.89 log reduction after irradiation at 2.1 W for 5 min. The temperature rise during aPDT/PACT was confirmed to be within a safe range. Furthermore, the light energy transmittance through the root was at a peak approximately 1 min after the start of irradiation, indicating that most of the ICG in the root canal was consumed. This study shows that aPDT/PACT can suppress E. faecalis in infected root canals with high efficiency.
- Subjects :
- 0301 basic medicine
indocyanine green
Swine
Root canal
medicine.medical_treatment
Photodynamic therapy
antimicrobial photodynamic therapy
Enterococcus faecalis
chemistry.chemical_compound
0302 clinical medicine
Antimicrobial chemotherapy
Biology (General)
Spectroscopy
Photosensitizing Agents
biology
Chemistry
General Medicine
Antimicrobial
Computer Science Applications
medicine.anatomical_structure
Lasers, Semiconductor
Nanospheres
030103 biophysics
QH301-705.5
Article
Catalysis
Microbiology
Inorganic Chemistry
03 medical and health sciences
medicine
Animals
Humans
Physical and Theoretical Chemistry
QD1-999
Molecular Biology
Gram-Positive Bacterial Infections
Periodontitis
Organic Chemistry
Biofilm
030206 dentistry
biology.organism_classification
medicine.disease
endodontics
Photochemotherapy
Biofilms
Indocyanine green
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....5c390a252436cf701759c7d4a38a84c8