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Label-free aptasensor for the detection of cardiac biomarker myoglobin based on gold nanoparticles decorated boron nitride nanosheets
- Source :
- Biosensors and Bioelectronics. 126:143-150
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- A novel electrochemical aptasensor based on gold nanoparticles decorated on boron nitride nanosheets (AuNPs/BNNSs) for the sensitive and selective detection of myoglobin (Mb) is reported. BNNSs were chemically synthesized by a low-cost and simple hydrothermal method. They were deposited onto the fluorine-doped tin oxide (FTO) electrode by a spin-coating method. Subsequently, AuNPs were chemically deposited onto the BNNS/FTO electrode by a seed-mediated chemical reduction method, with an average particle size of approximately 10 nm. The AuNPs/BNNSs/FTO electrode was used as a transducer to immobilize a thiol-functionalized DNA aptamer (Apt) via the covalent interaction of Au–S for the specific binding of Mb. [Fe(CN)6]3-/4- was used as a redox probe to monitor the oxidation current variation upon the binding of Mb with varying concentrations onto the sensor surface. The Apt/AuNPs/BNNSs/FTO sensor shows a high signal response for Mb with a detection limit of 34.6 ng/mL and a dynamic response range of 0.1–100 µg/mL. It is a promising candidate for point-of-care diagnosis in real samples. This strategy could make possible the application of other 2D materials with wide bandgaps for the development of biosensors.
- Subjects :
- Boron Compounds
Materials science
Point-of-Care Systems
Aptamer
Biomedical Engineering
Biophysics
Metal Nanoparticles
Biosensing Techniques
02 engineering and technology
Electrochemistry
01 natural sciences
chemistry.chemical_compound
Limit of Detection
Humans
Detection limit
Myoglobin
010401 analytical chemistry
Electrochemical Techniques
General Medicine
Aptamers, Nucleotide
021001 nanoscience & nanotechnology
Tin oxide
Nanostructures
0104 chemical sciences
Chemical engineering
chemistry
Boron nitride
Colloidal gold
Electrode
Gold
0210 nano-technology
Biosensor
Biotechnology
Subjects
Details
- ISSN :
- 09565663
- Volume :
- 126
- Database :
- OpenAIRE
- Journal :
- Biosensors and Bioelectronics
- Accession number :
- edsair.doi.dedup.....5c6382d0dcbe50d20aadb14a7dafe3f7