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Neurotoxic Effects of Stanozolol on Male Rats‘ Hippocampi: Does Stanozolol cause apoptosis?
- Source :
- Biomolecular Concepts, Vol 10, Iss 1, Pp 73-81 (2019)
- Publication Year :
- 2019
- Publisher :
- Walter de Gruyter GmbH, 2019.
-
Abstract
- Stanozolol is an anabolic-androgenic steroid which is commonly abused by athletes for improved energy, appearance, and physical size. It has been previously shown to cause changes in behaviour and has various physical effects. Studies have previously been conducted on its neurotoxic effect on the central nervous system (CNS), which are typically psychological in nature. This study was performed to investigate the apoptotic effect of stanozolol on different parts of the rat hippocampus. Sixteen male Wistar rats were divided randomly into two groups (experimental and control). The experimental group received subcutaneous injections of stanozolol (5mg/kg/day) for consecutive 28 days, whereas the control group received saline using the same dosing schedule and administration route. After routine procedures, coronal sections of rat brain were stained with Toluidine blue and TUNEL for pre-apoptotic and apoptotic cell detection, respectively. In order to compare groups, the mean number of TUNEL-positive and pre-apoptotic neurons per unit area were calculated and analysed. Histopathological examination revealed that the mean number of pre-apoptotic and apoptotic neurons in the CA1, CA2, CA3 and DG areas of the hippocampus were significantly increased in the stanozolol treated group. In conclusion, stanozolol abuse may induce pre-apoptotic and apoptotic cell formation in different regions of the hippocampus.
- Subjects :
- Male
medicine.medical_specialty
hippocampus
QH301-705.5
medicine.medical_treatment
Central nervous system
Apoptosis
010501 environmental sciences
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Cellular and Molecular Neuroscience
Anabolic Agents
0302 clinical medicine
Internal medicine
Male rats
medicine
Animals
Hippocampus (mythology)
Rats, Wistar
pre-apoptotic cells
Biology (General)
Saline
Stanozolol
anabolic-androgenic steroids
0105 earth and related environmental sciences
TUNEL assay
business.industry
apoptotic cells
General Medicine
stanozolol
Rat brain
Rats
medicine.anatomical_structure
Endocrinology
Androgens
business
030217 neurology & neurosurgery
medicine.drug
Subjects
Details
- ISSN :
- 1868503X and 18685021
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Biomolecular Concepts
- Accession number :
- edsair.doi.dedup.....5cc53da4b63f8004c6085d6c3c6801a9