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Evodiamine inhibits RANKL‐induced osteoclastogenesis and prevents ovariectomy‐induced bone loss in mice
- Source :
- Journal of Cellular and Molecular Medicine
- Publication Year :
- 2018
- Publisher :
- John Wiley and Sons Inc., 2018.
-
Abstract
- Postmenopausal osteoporosis (PMO) is a progressive bone disease characterized by the over‐production and activation of osteoclasts in elderly women. In our study, we investigated the anti‐osteoclastogenic effect of evodiamine (EVO) in vivo and in vitro, as well as the underlying mechanism. By using an in vitro bone marrow macrophage (BMM)‐derived osteoclast culture system, we found that EVO inhibited osteoclast formation, hydroxyapatite resorption and receptor activator of NF‐κB ligand (RANKL)‐induced osteoclast marker gene and protein expression. Mechanistically, we found that EVO inhibited the degradation and RANKL‐induced transcriptional activity of IκBα. RANKL‐induced Ca2+ oscillations were also abrogated by EVO. In vivo, an ovariectomized (OVX) mouse model was established to mimic PMO, and OVX mice received oral administration of either EVO (10 mg/kg) or saline every other day. We found that EVO can attenuate bone loss in OVX mice by inhibiting osteoclastogenesis. Taken together, our findings suggest that EVO suppresses RANKL‐induced osteoclastogenesis through NF‐κB and calcium signalling pathways and has potential value as a therapeutic agent for PMO.
- Subjects :
- 0301 basic medicine
musculoskeletal diseases
medicine.medical_specialty
Bone disease
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
In vivo
Osteoclast
Evodiamine
Internal medicine
medicine
biology
NF‐κB
Cell Biology
Original Articles
Ca2+ oscillation
medicine.disease
osteoporosis
3. Good health
Resorption
IκBα
030104 developmental biology
Endocrinology
medicine.anatomical_structure
evodiamine
ovariectomy
chemistry
RANKL
030220 oncology & carcinogenesis
osteoclast
Ovariectomized rat
biology.protein
Molecular Medicine
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 15824934 and 15821838
- Volume :
- 23
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine
- Accession number :
- edsair.doi.dedup.....5ccedbff01030ad3ac0bbfe5235c0813