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Colon carcinoma cells induce CXCL11-dependent migration of CXCR3-expressing cytotoxic T lymphocytes in organotypic culture
- Source :
- Cancer Immunology, Immunotherapy. 56:359-370
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Adoptive immunotherapy of cancer patients with cytolytic T lymphocytes (CTL) has been hampered by the inability of the CTL to home into tumors in vivo. Chemokines can attract T lymphocytes to the tumor site, as demonstrated in animal models, but the role of chemokines in T-lymphocyte trafficking toward human tumor cells is relatively unexplored. In the present study, the role of chemokines and their receptors in the migration of a colon carcinoma (CC) patient's CTL toward autologous tumor cells has been studied in a novel three-dimensional organotypic CC culture. CTL migration was mediated by chemokine receptor CXCR3 expressed by the CTL and CXCL11 chemokine secreted by the tumor cells. Excess CXCL11 or antibodies to CXCL11 or CXCR3 inhibited migration of CTL to tumor cells. T cell and tumor cell analyses for CXCR3 and CXCL11 expression, respectively, in ten additional CC samples, may suggest their involvement in other CC patients. Our studies, together with previous studies indicating angiostatic activity of CXCL11, suggest that CXCL11 may be useful as an immunotherapeutic agent for cancer patients when transduced into tumor cells or fused to tumor antigen-specific Ab.
- Subjects :
- Cancer Research
Chemokine
Receptors, CXCR3
T cell
Immunology
Apoptosis
Enzyme-Linked Immunosorbent Assay
chemical and pharmacologic phenomena
Biology
CXCR3
Sensitivity and Specificity
Tissue Culture Techniques
Cell Movement
Cell Line, Tumor
parasitic diseases
medicine
Humans
Immunology and Allergy
Cytotoxic T cell
CXCL11
Reverse Transcriptase Polymerase Chain Reaction
hemic and immune systems
Chemokine CXCL11
CTL
Phenotype
medicine.anatomical_structure
Oncology
Cell culture
Colonic Neoplasms
biology.protein
Cytokines
Female
Receptors, Chemokine
CC chemokine receptors
Chemokines, CXC
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 14320851 and 03407004
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Cancer Immunology, Immunotherapy
- Accession number :
- edsair.doi.dedup.....5cfba5d1a2805a8dae16b5da15e71341
- Full Text :
- https://doi.org/10.1007/s00262-006-0190-2