Back to Search
Start Over
Improvement of Hepatitis C Virus (HCV) Genotype Determinationwith the New Version of the INNO-LiPA HCV Assay
- Source :
- Journal of Clinical Microbiology, Journal of Clinical Microbiology, American Society for Microbiology, 2007, 45 (4), pp.1140-1145. ⟨10.1128/JCM.01982⟩, Journal of Clinical Microbiology, 2007, 45 (4), pp.1140-1145. ⟨10.1128/JCM.01982⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- Hepatitis C virus (HCV) isolates have been classified into six main genotypes. Genotyping methods, and especially the widely used line probe assay (LiPA), are frequently based on the 5′-untranslated region (5′UTR). However, this region is not appropriate for discriminating HCV strains at the subtype level and for distinguishing many genotype 6 samples from genotype 1. We investigated the capacity of a novel LiPA (Versant HCV Genotype 2.0 assay) based on the simultaneous detection of 5′UTR and Core regions for genotypes 1 and 6 to provide correct HCV genotypes (characterized with a phylogenetic analysis) in a set of HCV strains mainly encountered in Western countries. The improvement was assessed by comparing the results to those obtained with the previous version of the assay. Of the 135 tested samples, 64.7% were concordant for genotype group and subtype with sequencing reference results using the Versant HCV Genotype 2.0 assay versus 37.5% with the previous version. The yield was mainly related to a better characterization of genotype 1, since the accuracy, tested in 62 genotype 1 samples, increased from 45.2% with the first version to 96.8% with the new one. However, this new version necessitates a specific PCR and could no longer be used after 5′UTR PCR used for current HCV infection diagnosis. Moreover, the information provided by 5′UTR hybridization is not reliable for correctly identifying the diversity within genotypes 2 and 4. Thus, the Versant HCV Genotype 2.0 assay remains a useful tool for clinical practice when only the discrimination between major HCV genotypes is necessary.
- Subjects :
- Microbiology (medical)
Genotype
Hepacivirus
Hepatitis C virus
[SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology
Viral Nonstructural Proteins
medicine.disease_cause
Polymerase Chain Reaction
Virus
law.invention
03 medical and health sciences
Flaviviridae
0302 clinical medicine
law
Predictive Value of Tests
Virology
medicine
Humans
Genotyping
Polymerase chain reaction
Genetics
0303 health sciences
biology
030306 microbiology
Viral Core Proteins
Nucleic Acid Hybridization
Hepatitis C
Sequence Analysis, DNA
medicine.disease
biology.organism_classification
3. Good health
Hepatitis C Virus (HCV) G
030211 gastroenterology & hepatology
5' Untranslated Regions
Subjects
Details
- Language :
- English
- ISSN :
- 00951137
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology, Journal of Clinical Microbiology, American Society for Microbiology, 2007, 45 (4), pp.1140-1145. ⟨10.1128/JCM.01982⟩, Journal of Clinical Microbiology, 2007, 45 (4), pp.1140-1145. ⟨10.1128/JCM.01982⟩
- Accession number :
- edsair.doi.dedup.....5d16b5921d089d16848f01f79030c777