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Study on the effect of citric acid adaptation toward the subsequent survival of Lactobacillus plantarum NCIMB 8826 in low pH fruit juices during refrigerated storage
Study on the effect of citric acid adaptation toward the subsequent survival of Lactobacillus plantarum NCIMB 8826 in low pH fruit juices during refrigerated storage
- Source :
- Food research international (Ottawa, Ont.). 111
- Publication Year :
- 2018
-
Abstract
- Pre-treatment of stationary phase cells of Lactobacillus plantarum NCMIB 8826 with citric acid (pH 3 to 6) for a short period of time significantly improved subsequent cell survival in several highly acidic fruit juices namely cranberry (pH 2.7), pomegranate (pH 3.5), and lemon & lime juices (pH 2.8). Although the mechanism for this adaptation is still unclear, the analysis of the cellular fatty acid content of acid adapted cells and the reverse transcription polymerase chain reaction (RT-PCR) showed a significant increase (by ~1.7 fold) of the cellular cyclopropane fatty acid, cis-11,12-methylene octadecanoic acid (C19:0cyclow7c) and a significant upregulation (~12 fold) of cyclopropane synthase (cfa) were observed, respectively, during acid adaptation. It is likely that these changes led to a decrease in membrane fluidity and to lower membrane permeability, which prevents the cells from proton influx during storage in these low pH fruit juices.
- Subjects :
- 0301 basic medicine
Citrus
Membrane permeability
030106 microbiology
Citric Acid
03 medical and health sciences
chemistry.chemical_compound
Refrigeration
Membrane fluidity
Cyclopropane fatty acid
Food science
chemistry.chemical_classification
Lythraceae
ATP synthase
biology
Fatty acid
Oxides
Calcium Compounds
Hydrogen-Ion Concentration
biology.organism_classification
Reverse transcription polymerase chain reaction
Fruit and Vegetable Juices
030104 developmental biology
Vaccinium macrocarpon
chemistry
Food Storage
biology.protein
Citric acid
Lactobacillus plantarum
Food Science
Subjects
Details
- ISSN :
- 18737145 and 09639969
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- Food research international (Ottawa, Ont.)
- Accession number :
- edsair.doi.dedup.....5d560f398ca6d8a9cd580f42d92baf45