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Pulmonary vascular changes in extremely preterm sheep after intra-amniotic exposure to Ureaplasma parvum and lipopolysaccharide
- Source :
- PloS one, vol 12, iss 6, PLoS ONE, PLoS ONE, Vol 12, Iss 6, p e0180114 (2017), PLOS ONE, 12(6):0180114. Public Library of Science
- Publication Year :
- 2017
- Publisher :
- eScholarship, University of California, 2017.
-
Abstract
- Background\ud \ud Chorioamnionitis can induce pulmonary inflammation and promote bronchopulmonary dysplasia development, distinguished by alveolar simplification and impaired vascular growth. Chorioamnionitis is more common during the extremely preterm canalicular lung stage (crucial for vascular development); and increases the risk for subsequent sepsis. We hypothesized that single/combined exposure to chronic and/or acute inflammation induces pulmonary inflammatory responses and vascular changes.\ud \ud Methods\ud \ud Ovine fetuses were intra-amniotically exposed to chronic Ureaplasma parvum (UP) at 24 days (d) before extreme preterm delivery at 94d (term 147d) and/or to lipopolysaccharide (LPS) 7 or 2d before delivery. Pulmonary inflammation, vascular remodeling and angiogenic factors were assessed.\ud \ud Results\ud \ud LPS exposure increased CD3-positive and myeloperoxidase-positive cells. Combined UP-LPS exposure increased pulmonary inflammation compared with 2d LPS or UP groups. The UP+2d LPS group had an increased adventitial fibrosis score when compared with UP-treated animals. A reduced wall-to-lumen ratio was found in the 7d LPS animals when compared to the 2d LPS-treated animals. Exposure to UP+2d LPS reduced VEGF and VEGFR-2 levels compared with 2d LPS-treated animals. Angiopoietin-1 (Ang1) and tunica interna endothelial cell kinase 2 (Tie-2) levels were decreased after UP+7d LPS as well as after 7d LPS, but not with UP alone.\ud \ud Conclusion\ud \ud Chronic UP and subsequent LPS exposure increased pulmonary inflammation and decreased expression of angiogenic growth factors and receptors when compared to single hit-exposed animals.\ud \ud Figures
- Subjects :
- 0301 basic medicine
Lipopolysaccharides
FETAL SHEEP
Physiology
Maternal Health
lcsh:Medicine
Chorioamnionitis
Pathology and Laboratory Medicine
Toxicology
Low Birth Weight and Health of the Newborn
Ureaplasma
0302 clinical medicine
Endocrinology
Fibrosis
Models
Pregnancy
Infant Mortality
Medicine and Health Sciences
Toxins
lcsh:Science
Immune Response
Lung
Mammals
Pediatric
Multidisciplinary
biology
OVINE MODEL
TRAINED IMMUNITY
Obstetrics and Gynecology
Ruminants
Arterioles
medicine.anatomical_structure
Infectious Diseases
Maternal Exposure
Vertebrates
Models, Animal
Respiratory
lipids (amino acids, peptides, and proteins)
Female
medicine.symptom
Anatomy
Research Article
Endothelium
General Science & Technology
Immunology
Toxic Agents
Bacterial Toxins
Inflammation
Preterm Birth
Andrology
Sepsis
03 medical and health sciences
Signs and Symptoms
Extraction techniques
Diagnostic Medicine
Preterm
030225 pediatrics
Growth Factors
medicine
Animals
Fetus
Sheep
HYPERTENSION
Endocrine Physiology
Animal
lcsh:R
Organisms
Biology and Life Sciences
BRONCHOPULMONARY DYSPLASIA
Perinatal Period - Conditions Originating in Perinatal Period
medicine.disease
biology.organism_classification
RNA extraction
Endotoxins
Pregnancy Complications
Research and analysis methods
030104 developmental biology
ENDOTHELIAL GROWTH-FACTOR
Amniotes
Birth
Cardiovascular Anatomy
Women's Health
Blood Vessels
ENDOTOXIN
lcsh:Q
Subjects
Details
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PloS one, vol 12, iss 6, PLoS ONE, PLoS ONE, Vol 12, Iss 6, p e0180114 (2017), PLOS ONE, 12(6):0180114. Public Library of Science
- Accession number :
- edsair.doi.dedup.....5d991868b6450acce6a8bd44e80371dd