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The Use of MALDI-TOF Mass Spectrometry to Analyze Commensal Oral Yeasts in Nursing Home Residents
- Source :
- Microorganisms, Microorganisms, Vol 9, Iss 142, p 142 (2021), Volume 9, Issue 1
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid and accurate method to identify microorganisms in clinical laboratories. This study isolates yeast-like microorganisms in the oral washes that are collected from non-bedridden nursing home residents, using CHROMagar Candida plates, and identifies them using Bruker MALDI-TOF MS. The ribosomal DNA sequences of the isolates are then examined. Three hundred and twenty yeast isolates are isolated from the oral washes. Candida species form the majority (78.1%), followed by Trichosporon/Cutaneotrichosporon species (8.8%). Bruker MALDI-TOF MS gives a high-level confidence, with a log(score) value of &ge<br />1.8, and identifies 96.9% of the isolates. There are six inconclusive results (1.9%), and those sequences are verified as rare clinical species, including Candida ethanolica, Cutaneotrichosporon jirovecii, Exophiala dermatitidis, and Fereydounia khargensis. Almost all of the isolates have a regular color on the CHROMagar Candida plates. If the colonies are grouped by color on the plates, a specific dominant yeast species is present in each color group, except for purple or orange isolates. In conclusion, MALDI-TOF MS is verified as a fast, accurate and practical method to analyze oral yeasts in elderly subjects.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
030106 microbiology
oral cavity microorganisms
Microbiology
Article
03 medical and health sciences
Virology
Trichosporon
CHROMagar Candida
MALDI-TOF MS
lcsh:QH301-705.5
Ribosomal DNA
biology
geriatric medicine
biology.organism_classification
MALDI-TOF Mass Spectrometry
Yeast
Matrix-assisted laser desorption/ionization
030104 developmental biology
lcsh:Biology (General)
Chromagar candida
commensal yeast
Nursing homes
Exophiala dermatitidis
Subjects
Details
- ISSN :
- 20762607
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Microorganisms
- Accession number :
- edsair.doi.dedup.....5da67f2bf178994222b6e7c37b56692a