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Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis
- Source :
- Proceedings of the National Academy of Sciences of the United States of America (Online) (2004)., info:cnr-pdr/source/autori:I Zucchi 1, E Mento, V A Kuznetsov, M Scotti, V Valsecchi, B Simionati, E Vicinanza, G Valle, S Pilotti, R Reinbold, P Vezzoni, A Albertini, R Dulbecco/titolo:Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis/doi:/rivista:Proceedings of the National Academy of Sciences of the United States of America (Online)/anno:2004/pagina_da:/pagina_a:/intervallo_pagine:/volume, Proceedings of the National Academy of Sciences of the United States of America 101 (2004): 18147–18152. doi:10.1073/pnas.0408260101, info:cnr-pdr/source/autori:Zucchi I, Mento E, Kuznetsov VA, Scotti M, Valsecchi V, Simionati B, Vicinanza E, Valle G, Pilotti S, Reinbold R, Vezzoni P, Albertini A, Dulbecco R./titolo:Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis/doi:10.1073%2Fpnas.0408260101/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2004/pagina_da:18147/pagina_a:18152/intervallo_pagine:18147–18152/volume:101
- Publication Year :
- 2004
- Publisher :
- Proceedings of the National Academy of Sciences, 2004.
-
Abstract
- Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto , which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.
- Subjects :
- DNA, Complementary
Down-Regulation
Breast Neoplasms
In situ hybridization
Biology
Epithelium
breast cancer
Breast cancer
Gene expression
medicine
Carcinoma
Humans
Breast
Serial analysis of gene expression
Gene
In Situ Hybridization
Gene Library
Multidisciplinary
Cancer
Epithelial Cells
Biological Sciences
medicine.disease
Up-Regulation
Gene Expression Regulation, Neoplastic
medicine.anatomical_structure
Lymphatic Metastasis
Immunology
Cancer research
seriall analysis of gene expression
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....5daf0387909fddc3e9ab9c697861089e
- Full Text :
- https://doi.org/10.1073/pnas.0408260101