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Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis

Authors :
E. Mento
Alberto Albertini
V. Valsecchi
Barbara Simionati
S. Pilotti
Maddalena Scotti
Vladimir A. Kuznetsov
Rolland Reinbold
Renato Dulbecco
Paolo Vezzoni
Ileana Zucchi
E. Vicinanza
Giorgio Valle
Source :
Proceedings of the National Academy of Sciences of the United States of America (Online) (2004)., info:cnr-pdr/source/autori:I Zucchi 1, E Mento, V A Kuznetsov, M Scotti, V Valsecchi, B Simionati, E Vicinanza, G Valle, S Pilotti, R Reinbold, P Vezzoni, A Albertini, R Dulbecco/titolo:Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis/doi:/rivista:Proceedings of the National Academy of Sciences of the United States of America (Online)/anno:2004/pagina_da:/pagina_a:/intervallo_pagine:/volume, Proceedings of the National Academy of Sciences of the United States of America 101 (2004): 18147–18152. doi:10.1073/pnas.0408260101, info:cnr-pdr/source/autori:Zucchi I, Mento E, Kuznetsov VA, Scotti M, Valsecchi V, Simionati B, Vicinanza E, Valle G, Pilotti S, Reinbold R, Vezzoni P, Albertini A, Dulbecco R./titolo:Gene expression profiles of epithelial cells microscopically isolated from a breast-invasive ductal carcinoma and a nodal metastasis/doi:10.1073%2Fpnas.0408260101/rivista:Proceedings of the National Academy of Sciences of the United States of America/anno:2004/pagina_da:18147/pagina_a:18152/intervallo_pagine:18147–18152/volume:101
Publication Year :
2004
Publisher :
Proceedings of the National Academy of Sciences, 2004.

Abstract

Expression profiles of breast carcinomas are difficult to interpret when they are obtained from tissue in toto , which may contain a large proportion of non-cancer cells. To avoid this problem, we microscopically isolated cells from a primary invasive ductal carcinoma of the breast and from an axillary node harboring a metastatic breast carcinoma, to obtain pure populations of carcinoma cells (≈500) and used them for serial analysis of gene expression. The expression profiles generated from both populations of cells were compared with the profile of a disease-free mammary epithelium. We showed that the expression profiles obtained are exclusive of carcinoma cells with no contribution of non-epithelial cells. From a total of 16,939 unique tags analyzed, we detected 559 statistically significant changes in gene expression; some of these genes have not been previously associated with breast cancer. We observed that many of the down-regulated genes are the same in both cancers, whereas the up-regulated genes are completely different, suggesting that the down-regulation of a set of genes may be the basic mechanism of cancer formation, while the up-regulation may characterize and possibly control the state of evolution of individual cancers. The results obtained may help in characterizing the neoplastic process of breast cancer.

Details

ISSN :
10916490 and 00278424
Volume :
101
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences
Accession number :
edsair.doi.dedup.....5daf0387909fddc3e9ab9c697861089e
Full Text :
https://doi.org/10.1073/pnas.0408260101