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Establishment of a canine model of acute pulmonary embolism with definite right ventricular dysfunction through introduced autologous blood clots
- Source :
- Thrombosis Research. 135:727-732
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- To establish a canine model of acute pulmonary embolism (PE) with right ventricular (RV) dysfunction using autologous blood clots and evaluate by echocardiography and contrast-enhanced Computed Tomography (CT).Autologous blood clots formed in vitro were introduced sequentially into the pulmonary arteries of eight healthy mixed-breed dogs while monitoring pulmonary and systemic hemodynamic function. Blood clots were injected until the mean pulmonary artery pressure (MPAP) reached two-three times the baseline pressure, which was maintained up to 1 hour. The RV function was assessed by echocardiography and ECG-gated dual-source contrast CT.All animals survived the imaging procedure. The post-injection pulmonary angiograms showed extensive PE, and MPAP increased from 16.50±2.45 mmHg to 43.13±4.91 mmHg (P0.001). On echocardiography, the RV fractional area change decreased from 42.06±3.36 to 27.96±3.54 (P0.001), and the RV myocardial performance increased from 0.20±0.05 to 0.63±0.16 (P0.001). On CT, the RV end-systolic volume increased from 11.11±1.81 ml to 24.71±4.60 ml (P0.001), RV end-diastolic volume from 20.73±2.83 ml to 34.63±5.76 ml (P0.001), and the four-chamber RV/left ventricular diameter ratio from 0.38±0.07 to 0.81±0.14 (P0.001).Acute PE with RV dysfunction was established in a large animal model through controlled injection of autologous blood clots, which may be useful for developing and evaluating new therapeutic approaches for acute PE with RV dysfunction.
- Subjects :
- medicine.medical_specialty
business.industry
Ventricular Dysfunction, Right
Autologous blood
Hemodynamics
Hematology
medicine.disease
Right ventricular dysfunction
Pulmonary embolism
Disease Models, Animal
Dogs
Echocardiography
Internal medicine
medicine.artery
Acute Disease
Rv function
Pulmonary artery
medicine
Cardiology
Animals
Pulmonary Embolism
Tomography, X-Ray Computed
business
Canine model
Large animal
Subjects
Details
- ISSN :
- 00493848
- Volume :
- 135
- Database :
- OpenAIRE
- Journal :
- Thrombosis Research
- Accession number :
- edsair.doi.dedup.....5dd4cf613180a0238e6ed0241f1f5ed8