Back to Search
Start Over
The linkage specificity determination of Ube2g2-gp78 mediated polyubiquitination
- Source :
- Biochemical and Biophysical Research Communications. 473:1139-1143
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Polyubiquitin chain linkage specificity or topology is essential for its role in diverse cellular processes. Previous studies pay more attentions to the linkage specificity of the first ubiquitin moieties, whereas, little is known about the editing mechanism of linkage specificity in longer polyubiquitin chains. gp78 and its cognate E2-Ube2g2 catalyze lysine48 (K48)-linked polyubiquitin chains to promote the degradation of targeted proteins. Here, we show that the linkage specificity of the entire polyubiquitin chain is determined by the conjugation manner of the first ubiquitin molecule but not the following ones. Further study discovered that the gp78 CUE domain works as a proofreading machine during the growth of K48-linked polyubiquitin chains to ensure the linkage specificity. Together, our studies uncover a novel mechanism underlying the linkage specificity determination of longer polyubiquitin chains.
- Subjects :
- 0301 basic medicine
Biophysics
macromolecular substances
Plasma protein binding
Computational biology
Linkage (mechanical)
Ubiquitin-conjugating enzyme
environment and public health
Biochemistry
law.invention
Structure-Activity Relationship
03 medical and health sciences
Enzyme activator
Ubiquitin
law
Structure–activity relationship
Binding site
Polyubiquitin
Molecular Biology
Binding Sites
030102 biochemistry & molecular biology
biology
Cell Biology
Enzyme Activation
Receptors, Autocrine Motility Factor
030104 developmental biology
Amino Acid Substitution
Ubiquitin-Conjugating Enzymes
biology.protein
Proofreading
Protein Binding
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 473
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....5de2c38ec069621af116dfb4f5880403