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Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry
- Source :
- International Journal of Nanomedicine
- Publication Year :
- 2015
- Publisher :
- Dove Medical Press, 2015.
-
Abstract
- Yuri D Ivanov,1 Anna L Kaysheva,1,2 Pavel A Frantsuzov,1 Tatyana O Pleshakova,1 Nikolay V Krohin,1 Alexander A Izotov,1 Ivan D Shumov,1 Vasiliy F Uchaikin,1 Vladimir A Konev,1 Vadim S Ziborov,1 Alexander I Archakov11Institute of Biomedical Chemistry, 2PostgenTech Ltd, Moscow, RussiaAbstract: A method for detection and identification of core antigen of hepatitis C virus (HCVcoreAg)-containing particles in the serum was proposed, with due account taken of the interactions of proteotypic peptides with Na+, K+, and Cl- ions. The method is based on a combination of reversible biospecific atomic force microscopy (AFM)-fishing and mass spectrometry (MS). AFM-fishing enables concentration, detection, and counting of protein complexes captured on the AFM chip surface, with their subsequent MS identification. Biospecific AFM-fishing of HCVcoreAg-containing particles from serum samples was carried out using AFM chips with immobilized antibodies against HCVcoreAg (HCVcoreAgim). Formation of complexes between anti-HCVcoreAgim and HCVcoreAg-containing particles on the AFM chip surface during the fishing process was demonstrated. These complexes were registered and counted by AFM. Further MS analysis allowed reliable identification of HCVcoreAg within the complexes formed on the AFM chip surface. It was shown that MS data processing, with account taken of the interactions between HCVcoreAg peptides and Na+, K+ cations, and Cl- anions, allows an increase in the number of peptides identified.Keywords: hepatitis C virus, molecular detector, biospecific fishing
- Subjects :
- hepatitis C virus
Biophysics
Analytical chemistry
Pharmaceutical Science
Bioengineering
macromolecular substances
Mass spectrometry
Microscopy, Atomic Force
Mass Spectrometry
Ion
Biomaterials
International Journal of Nanomedicine
Drug Discovery
biospecific fishing
Humans
Hepatitis C virus core
Original Research
Atomic force microscopy
Chemistry
Viral Core Proteins
Organic Chemistry
Ms analysis
technology, industry, and agriculture
Immobilized Antibodies
molecular detector
General Medicine
Serum samples
Hepatitis C
Subjects
Details
- Language :
- English
- ISSN :
- 11782013 and 11769114
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi.dedup.....5de685f3b7e7ed772aa32c037f7c52cf