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Human Granulosa Cells: Insulin and Insulin-Like Growth Factor-1 Receptors and Aromatase Expression Modulation by Metformin
- Source :
- Gynecologic and Obstetric Investigation. 77:156-162
- Publication Year :
- 2014
- Publisher :
- S. Karger AG, 2014.
-
Abstract
- Background/Aim: Granulosa cells are the source of the most important ovarian steroids. Even in patients without significant improvement in metabolic parameters, metformin has apparently an important effect on the ovary. The aim of this study was to evaluate gene and protein expression of an insulin receptor (IR), insulin-like growth factor-1 (IGF1) receptor (IGF1R) and aromatase in granulosa cells treated with metformin and insulin. Methods: Luteinized granulosa cells were collected from 27 patients during in vitro fertilization procedures. Cells were isolated, stored in culture for 24 h and divided into four groups: control; metformin for 30 min, and metformin for 30 min plus insulin for 30 or 60 min. Results: IR and IGF1R mRNA expression was significantly enhanced by metformin but was not affected by insulin. Aromatase mRNA expression was significantly reduced in metformin-incubated cells following stimulation with insulin for 30 min. No statistical differences were found in IGF1R and aromatase protein expression, and IR expression was not detected. Conclusion: A direct effect of metformin on the gene expression of IGF1R, IR and aromatase was observed. Further studies should investigate the role of IGF1R, IR and aromatase in ovarian physiology for a better understanding of the effect of metformin.
- Subjects :
- Adult
endocrine system
medicine.medical_specialty
endocrine system diseases
medicine.medical_treatment
Gene Expression
Receptor, IGF Type 1
Insulin-like growth factor
Aromatase
Internal medicine
Gene expression
medicine
Protein biosynthesis
Humans
Hypoglycemic Agents
Insulin
RNA, Messenger
Receptor
Cells, Cultured
Granulosa Cells
biology
urogenital system
Chemistry
Obstetrics and Gynecology
Metformin
Receptor, Insulin
Insulin receptor
Endocrinology
Reproductive Medicine
Protein Biosynthesis
biology.protein
Female
medicine.drug
Subjects
Details
- ISSN :
- 1423002X and 03787346
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Gynecologic and Obstetric Investigation
- Accession number :
- edsair.doi.dedup.....5de8815d580c123814ccd4f5a5d0c495