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Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity
- Source :
- Nanotoxicology, Poulsen, S S, Jackson, P, Kling, K, Knudsen, K B, Skaug, V, Kyjovska, Z O, Thomsen, B L, Clausen, P A, Atluri, R, Berthing, T, Bengtson, S, Wolff, H, Jensen, K A, Wallin, H & Vogel, U B 2016, ' Multi-walled carbon nanotube physicochemical properties predict pulmonary inflammation and genotoxicity ', Nanotoxicology, vol. 10, no. 9, pp. 1263-1275 . https://doi.org/10.1080/17435390.2016.1202351
- Publication Year :
- 2016
-
Abstract
- Lung deposition of multi-walled carbon nanotubes (MWCNT) induces pulmonary toxicity. Commercial MWCNT vary greatly in physicochemical properties and consequently in biological effects. To identify determinants of MWCNT-induced toxicity, we analyzed the effects of pulmonary exposure to 10 commercial MWCNT (supplied in three groups of different dimensions, with one pristine and two/three surface modified in each group). We characterized morphology, chemical composition, surface area and functionalization levels. MWCNT were deposited in lungs of female C57BL/6J mice by intratracheal instillation of 0, 6, 18 or 54 μg/mouse. Pulmonary inflammation (neutrophil influx in bronchoalveolar lavage (BAL)) and genotoxicity were determined on day 1, 28 or 92. Histopathology of the lungs was performed on day 28 and 92. All MWCNT induced similar histological changes. Lymphocytic aggregates were detected for all MWCNT on day 28 and 92. Using adjusted, multiple regression analyses, inflammation and genotoxicity were related to dose, time and physicochemical properties. The specific surface area (BET) was identified as a positive predictor of pulmonary inflammation on all post-exposure days. In addition, length significantly predicted pulmonary inflammation, whereas surface oxidation (–OH and –COOH) was predictor of lowered inflammation on day 28. BET surface area, and therefore diameter, significantly predicted genotoxicity in BAL fluid cells and lung tissue such that lower BET surface area or correspondingly larger diameter was associated with increased genotoxicity. This study provides information on possible toxicity-driving physicochemical properties of MWCNT. The results may contribute to safe-by-design manufacturing of MWCNT, thereby minimizing adverse effects.
- Subjects :
- 0301 basic medicine
Pulmonary toxicity
Neutrophils
CNT-length
02 engineering and technology
Toxicology
medicine.disease_cause
Mice
Composite material
Lung
Inhalation exposure
Inhalation Exposure
medicine.diagnostic_test
CNT-diameter
021001 nanoscience & nanotechnology
3. Good health
medicine.anatomical_structure
Neutrophil Infiltration
Toxicity
Regression Analysis
functionalization
Female
Original Article
Comet Assay
0210 nano-technology
Bronchoalveolar Lavage Fluid
BET theory
Materials science
Surface Properties
Biomedical Engineering
Article
03 medical and health sciences
BET surface area
Specific surface area
medicine
Animals
Particle Size
Functionalization
lymphocytic aggregates
Dose-Response Relationship, Drug
Nanotubes, Carbon
030111 toxicology
DNA Breaks
Pneumonia
Molecular biology
Mice, Inbred C57BL
Bronchoalveolar lavage
Lymphocytic aggregates
Genotoxicity
Subjects
Details
- ISSN :
- 17435404
- Volume :
- 10
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Nanotoxicology
- Accession number :
- edsair.doi.dedup.....5df3e8d7ec6ed694d42796bcf982be62