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Clinicopathologic and Molecular Features of Paired Cases of Metachronous Ovarian Serous Borderline Tumor and Subsequent Serous Carcinoma
- Source :
- Am J Surg Pathol
- Publication Year :
- 2019
-
Abstract
- Although risk factors have been established for development of serous carcinoma after a diagnosis of serous borderline tumor (SBT), comprising atypical proliferative serous tumor (APST) [i.e. conventional SBT] and non-invasive low-grade serous carcinoma (niLGSC) [i.e., micropapillary SBT], subsequent invasive carcinoma still occurs in a subset of women that are not at increased risk. Whether subsequent serous carcinoma in women with a prior SBT represents malignant progression/recurrence or an independent primary tumor is unclear, and the combined clinicopathologic and molecular features of SBTs and their subsequent carcinomas have not been fully characterized. In this study, we analyzed a cohort of 42 women initially diagnosed with SBT who subsequently developed serous carcinoma out of a total of 1,025 cases of ovarian SBT from a nationwide population-based cohort. Review of the diagnostic slides was performed from this subset of SBTs and matched metachronous invasive serous carcinomas (39 low-grade, 3 high-grade). DNA was extracted from tissue blocks available for 41 cases (both SBT and carcinoma, n = 36; SBT only, n = 3; carcinoma only, n = 2). Samples were subjected to digital droplet PCR (ddPCR) to analyze mutation hotspots in KRAS (codon 12) and BRAF (V600E), which are frequently found in low-grade serous tumors. Eighty-one percent of SBTs (34/42) were APST, and 19% (8/42) were niLGSC. Forty percent of cases (17/42) were FIGO stage I, the majority of which were APST (14/17; 82%). The median time to development of carcinoma was 9 years (range, 0.6–25 years). Mutations in SBTs were distributed as follows: 5/39 (13%) BRAF-mutant, 22/39 (56%) KRAS-mutant, and 12/39 (31%) wild-type for both genes. There was a significant relationship between SBT gene mutation and histologic type, with BRAF-mutations occurring exclusively in APST and a higher frequency of niLGSC among SBTs wild-type for BRAF and KRAS (p = 0.01). The diffuse presence of tumor cells with abundant eosinophilic cytoplasm was significantly associated with the BRAF mutation (p = 0.001). Mutational analyses of matched SBT/carcinoma pairs revealed concordant profiles in 33/36 (92%) cases, of which 19 (53%) were KRAS-mutant, 4 (11%) were BRAF-mutant, and 10 (28%) were wild-type for both genes. The 3 discordant cases consisted of a wild-type niLGSC with a subsequent BRAF-mutant invasive LGSC, a KRAS(G12V)-mutant APST with a KRAS(G12C)-mutant LGSC, and a BRAF-mutant APST with subsequent development of a KRAS(G12D)-mutant high-grade serous carcinoma. In conclusion, some women with SBTs can subsequently develop serous carcinoma, occasionally over 10 years later. Most subsequent carcinomas are low grade, but a small subset can be high grade. The type of gene mutation of the SBT correlates with various histologic features. While most cases of serous carcinoma developing after a diagnosis of SBT probably represent tumor progression, a minority are independent primary tumors, presumably arising from endosalpingiosis.
- Subjects :
- 0301 basic medicine
Adult
Proto-Oncogene Proteins B-raf
medicine.medical_specialty
endocrine system diseases
Serous carcinoma
Population
DNA Mutational Analysis
Gene mutation
medicine.disease_cause
Gastroenterology
Article
Pathology and Forensic Medicine
Proto-Oncogene Proteins p21(ras)
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
Carcinoma
Biomarkers, Tumor
Humans
Registries
education
Genetic Association Studies
Aged
Ovarian Neoplasms
education.field_of_study
business.industry
Cystadenoma, Serous
Neoplasms, Second Primary
Middle Aged
medicine.disease
Primary tumor
Cystadenocarcinoma, Serous
Serous fluid
030104 developmental biology
030220 oncology & carcinogenesis
Mutation
Disease Progression
Surgery
Female
KRAS
Anatomy
Neoplasm Grading
business
Precancerous Conditions
V600E
Follow-Up Studies
Subjects
Details
- ISSN :
- 15320979
- Volume :
- 43
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- The American journal of surgical pathology
- Accession number :
- edsair.doi.dedup.....5df68d7018c8e4daa103b0c0e2665901