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Ferulic acid, an efficient inhibitor of type B trichothecene biosynthesis and Tri gene expression in Fusarium liquid cultures
- Source :
- Mycological Research, Mycological Research, Elsevier, 2009, 113 (6-7), pp.746-753. ⟨10.1016/j.mycres.2009.02.010⟩
- Publication Year :
- 2009
- Publisher :
- Elsevier BV, 2009.
-
Abstract
- International audience; The effect of ferulic acid, the most abundant phenolic acid in wheat bran, was studied in vitro on type B trichothecene biosynthesis by Fusarium. It was demonstrated that ferulic acid is an efficient inhibitor of mycotoxin production by all strains of Fusarium tested, including different chemotypes and species. To analyse the mechanism of toxin biosynthesis inhibition by ferulic acid, expression of representative Tri genes, involved in the trichothecene biosynthesis pathway, was monitored by real-time RT-PCR. A decrease in the level of Tri gene expression was measured, suggesting that inhibition of toxin synthesis by ferulic acid could be regulated at the transcriptional level. Moreover, toxin production was shown to be reduced proportionally to the initial amount of ferulic acid added in the culture medium. Addition of ferulic acid either at the spore germination step or to a mycelial culture resulted in the same final inhibitory effect on mycotoxin accumulation. A cumulative inhibitory effect on trichothecene biosynthesis was even observed with successive supplementation of ferulic acid. Ferulic acid, which content varies among wheat varieties, could then play an important role in modulating trichothecene biosynthesis by Fusarium in some wheat varieties.
- Subjects :
- Fusarium
Coumaric Acids
Trichothecene
MYCOTOXIN
Gene Expression
Plant Science
Fungal Proteins
Ferulic acid
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Culture Techniques
Genetics
Spore germination
PHENOLIC ACID
Mycotoxin
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
2. Zero hunger
0303 health sciences
biology
Bran
030306 microbiology
food and beverages
Phenolic acid
biology.organism_classification
Biosynthetic Pathways
TRI GENES
chemistry
Biochemistry
REGULATION
Trichothecenes
Biotechnology
Subjects
Details
- ISSN :
- 09537562 and 14698102
- Volume :
- 113
- Database :
- OpenAIRE
- Journal :
- Mycological Research
- Accession number :
- edsair.doi.dedup.....5e42b19b43d4df8d0b39690e9b99b62b