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Anti-oomycetes and immunostimulatory activity of natural plant extract compounds against Saprolegnia spp.: Molecular docking and in-vitro studies
- Source :
- Fishshellfish immunology. 114
- Publication Year :
- 2020
-
Abstract
- This study aimed to investigate the effectiveness of five natural plant extract compounds Curcumin (CUR); Eugenol (EUG), Cinnamaldehyde (CIN), Stigmasterol (ST) and Morin (MOR), on two species of Saprolegnia; Saprolegnia parasitica and S. australis. Selective compounds were screened for the minimum inhibitory concentration, first for anti-oomycetes activity and then mycelium growth inhibition, spore germination inhibition and colonisation test. Nitric oxide production and myeloperoxidase activity of the compounds were tested in head kidney leukocytes of rainbow trout, Oncorhynchus mykiss to assess the immunostimulatory potential. Molecular docking of effective compounds was carried out with effector proteins of S. parasitica to investigate the target binding sites. Among all, CUR could completely inhibit zoospore production and significantly (p ≤ .05) inhibit hyphal growth at 16 mg l−1 against S. parasitica and S. australis. CIN at the concentration of 50 mg l−1 completely inhibited hyphal growth of both Saprolegnia spp., although the zoospore production of S. parasitica and S. australis was reduced at 25 mg l−1 and 10 mg l−1. In the case of EUG, significant inhibition of the hyphal growth and germination of S. parasitica zoospores was observed at 50 mg l−1. ST and MOR did not show antioomycetes activity. The molecular docking results were consistent with in vitro studies, possibly due to the binding with the vital proteins (Plasma membrane ATPase, V-type proton ATPase, TKL protein kinase, Host targeting protein 1) of S. parasitica and ultimately inhibiting their activity. CUR and CIN showed increased nitric oxide production at the highest concentration of 250 and 256 mg l−1 but the value was not significant (p ≤ .05) with control. CUR showed significantly higher peroxidase activity (p ≤ .05) at a concentration of 256 mg l−1 though values were significantly similar with concentration from 16 to 128 mg l−1. The nitric oxide and total peroxidase activity of rainbow trout leukocytes in the case of CIN showed a significant difference only at 250 mg l−1 against the control. The results conclude that CUR, CIN showed the better anti-Saprolegnia activity and could be used as phyto-additives in aquaculture. Among all, the inclusion of CUR as phyto-additives will provide additional immunostimulatory activity.
- Subjects :
- 0301 basic medicine
Hyphal growth
Cellular immunity
Curcumin
Zoospore
Cell Survival
Microbial Sensitivity Tests
Aquatic Science
Biology
Saprolegnia
03 medical and health sciences
chemistry.chemical_compound
Minimum inhibitory concentration
Eugenol
Spore germination
Leukocytes
Environmental Chemistry
Animals
Acrolein
Dose-Response Relationship, Drug
Plant Extracts
fungi
04 agricultural and veterinary sciences
General Medicine
biology.organism_classification
Head Kidney
Molecular Docking Simulation
030104 developmental biology
chemistry
Biochemistry
Oncorhynchus mykiss
040102 fisheries
biology.protein
0401 agriculture, forestry, and fisheries
Growth inhibition
Peroxidase
Subjects
Details
- ISSN :
- 10959947
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Fishshellfish immunology
- Accession number :
- edsair.doi.dedup.....5e4909460135c4dfadc57fe54a89c66b