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Intracellular glutathione levels affect the outcomes of verteporfin-mediated photodynamic therapy in esophageal cancer cells

Authors :
Mirai, Edano
Tsutomu, Kanda
Ryohei, Tarumoto
Wataru, Hamamoto
Takashi, Hasegawa
Yukari, Mae
Takumi, Onoyama
Tomoaki, Takata
Takaaki, Sugihara
Hajime, Isomoto
Source :
Photodiagnosis and Photodynamic Therapy. 40:103090
Publication Year :
2022
Publisher :
Elsevier BV, 2022.

Abstract

Photodynamic therapy (PDT) induces cancer cell death by generating reactive oxygen species (ROS). In this process, photosensitizers accumulate in cancer cells irradiated by laser light of a specific wavelength, leading to ROS generation. Verteporfin (VP), a second-generation photosensitizer, is used in PDT for age-related macular degeneration. However, the antitumor effects of VP-PDT remain poorly defined. This study investigated the antitumor effects of VP-PDT on esophageal cancer (EC) cell lines in vitro. Two types of EC cell lines, the KYSE30 cell line, derived from highly differentiated esophageal carcinoma, and the KYSE170 cell line, derived from moderately differentiated carcinoma, were used in this study. VP-PDT exerted effective anticancer effects in both cell lines. Our results revealed that the low-density lipoprotein receptor, albumin receptor, and heme carrier protein-1 in VP uptake were not involved in VP uptake. However, cells rich in intracellular glutathione were resistant to VP-PDT. Our study outcomes suggest that lowering intracellular glutathione via a glutathione synthesis inhibitor or sulfasalazine can increase the effectiveness of VP-PDT-mediated anticancer effects.

Details

ISSN :
15721000
Volume :
40
Database :
OpenAIRE
Journal :
Photodiagnosis and Photodynamic Therapy
Accession number :
edsair.doi.dedup.....5e49cf5ba2ad911400a4d70ad48faee5
Full Text :
https://doi.org/10.1016/j.pdpdt.2022.103090