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Evaluation of a point-of-care diagnostic to identify glucose-6-phosphate dehydrogenase deficiency in Brazil
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 15, Iss 8, p e0009649 (2021)
- Publication Year :
- 2021
- Publisher :
- Public Library of Science, 2021.
-
Abstract
- Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a common enzyme deficiency, prevalent in many malaria-endemic countries. G6PD-deficient individuals are susceptible to hemolysis during oxidative stress, which can occur from exposure to certain medications, including 8-aminoquinolines used to treat Plasmodium vivax malaria. Accordingly, access to point-of-care (POC) G6PD testing in Brazil is critical for safe treatment of P. vivax malaria. Methodology/Principal findings This study evaluated the performance of the semi-quantitative, POC STANDARD G6PD Test (SD Biosensor, Republic of Korea). Participants were recruited at clinics and through an enriched sample in Manaus and Porto Velho, Brazil. G6PD and hemoglobin measurements were obtained from capillary samples at the POC using the STANDARD and HemoCue 201+ (HemoCue AB, Sweden) tests. A thick blood slide was prepared for malaria microscopy. At the laboratories, the STANDARD and HemoCue tests were repeated on venous samples and a quantitative spectrophotometric G6PD reference assay was performed (Pointe Scientific, Canton, MI). G6PD was also assessed by fluorescent spot test. In Manaus, a complete blood count was performed. Samples were analyzed from 1,736 participants. In comparison to spectrophotometry, the STANDARD G6PD Test performed equivalently in determining G6PD status in venous and capillary specimens under varied operating temperatures. Using the manufacturer-recommended reference value thresholds, the test’s sensitivity at the<br />Author summary G6PD deficiency affects an estimated 500 million people worldwide and is prevalent in many malaria-endemic settings. People with G6PD deficiency are at risk of hemolysis when exposed to certain medications, including 8-aminoquinoline drugs used to treat Plasmodium vivax malaria. Increased access to testing for G6PD deficiency at or near the point of care is critical for expanding the safe treatment of P. vivax malaria. In this study, we aimed to evaluate the performance of a point-of-care, semi-quantitative test for G6PD deficiency, the STANDARD G6PD Test, in a malaria-endemic setting in Brazil. The test was evaluated on both capillary and venous blood samples across a broad range of operating temperatures. The findings show that the STANDARD G6PD Test performed equivalently to the reference test in its ability to diagnose G6PD deficiency at the point of care. The STANDARD G6PD Test is a promising tool to aid in detecting G6PD deficiency at the point of care in Brazil.
- Subjects :
- Male
Plasmodium
Physiology
RC955-962
Biosensing Techniques
Gastroenterology
Biochemistry
Geographical locations
Medical Conditions
Arctic medicine. Tropical medicine
Medicine and Health Sciences
Child
Glucose-6-Phosphate Dehydrogenase Deficiency
Aged, 80 and over
medicine.diagnostic_test
Complete blood count
Anemia
Hematology
Middle Aged
Body Fluids
Infectious Diseases
Blood
Point-of-Care Testing
Child, Preschool
Aminoquinolines
Female
Public aspects of medicine
RA1-1270
Anatomy
Brazil
Research Article
Adult
medicine.medical_specialty
Adolescent
Blood slide
Glucosephosphate Dehydrogenase
Hemolysis
Antimalarials
Young Adult
Internal medicine
parasitic diseases
Parasite Groups
medicine
Parasitic Diseases
Malaria, Vivax
Humans
Hemoglobin
Point of care
Aged
business.industry
Public Health, Environmental and Occupational Health
Hemolytic Anemia
Biology and Life Sciences
Proteins
South America
medicine.disease
Tropical Diseases
Confidence interval
Malaria
Capillaries
Blood Counts
Cross-Sectional Studies
Glucosephosphate Dehydrogenase Deficiency
ROC Curve
Vivax malaria
Cardiovascular Anatomy
Linear Models
Blood Vessels
Parasitology
People and places
business
Apicomplexa
Glucose-6-phosphate dehydrogenase deficiency
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 15
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....5e4c51970e049b7b5d181f14c35eabd3