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Twelve novel FBN1 mutations in Marfan syndrome and Marfan related phenotypes test the feasibility of FBN1 mutation testing in clinical practice
- Publication Year :
- 2016
-
Abstract
- Marfan syndrome (MFS) is one of the major heritable disorders of connective tissue with a prevalence of between 1 in 5-10 000.1,2 It is characterised by features in the cardiovascular, ocular, and musculoskeletal systems and the Ghent criteria form a useful framework for its diagnosis.3 Mutations in FBN1 encoding the extracellular matrix protein fibrillin-1 classically cause MFS.4 Fibrillin-1, comprising multiple repeating subunits, of which the most common is the calcium binding epidermal growth factor (cbEGF) domain, is a key component of 10-12 nm microfibrils.5 Mutation detection has not been performed routinely in MFS because of the size and complexity of FBN1, which contains 65 exons extending over 200 kb of genomic DNA.5,6 Mutations are nearly always specific to each family and to date ∼300 mutations have been reported.7–10 FBN1 mutations have been found in 20-80% of patients with MFS depending upon the clinical selection of patients and the mutation detection method used.11–17 FBN1 mutations have been reported in a wide range of phenotypes in the Marfan spectrum1; neonatal MFS is associated with some FBN1 mutations in exons 24-3218; mutations of various types throughout FBN1 have been associated with classical MFS8; and FBN1 mutations have also been reported in many of the non-classical Marfan related phenotypes.1 The Ghent criteria propose that an FBN1 mutation is only of help diagnostically if it has been previously found in a person who independently meets the criteria for diagnosis of MFS.3 Other than mutations causing neonatal MFS, which occur in exons 24-32, correlation between genotype and phenotype is poor. The majority of patients with cysteine substitutions have classical MFS, and cysteine substitutions in exons 26-32 appear to be associated with classical disease manifesting early in life. …
- Subjects :
- Adult
musculoskeletal diseases
Marfan syndrome
congenital, hereditary, and neonatal diseases and abnormalities
Adolescent
Fibrillin-1
DNA Mutational Analysis
Heteroduplex Analysis
Biology
Fibrillins
Nucleic Acid Denaturation
medicine.disease_cause
Marfan Syndrome
Exon
Genotype-phenotype distinction
Gene duplication
Genetics
medicine
Humans
Child
skin and connective tissue diseases
Chromatography, High Pressure Liquid
Genetics (clinical)
Mutation
Microfilament Proteins
Gene Amplification
Middle Aged
medicine.disease
Phenotype
Mutation testing
Nucleic Acid Conformation
Electrophoresis, Polyacrylamide Gel
Fibrillin
Letter to JMG
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....5e64b31976e33b44d59b66632461de1a