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Effects of high hydrostatic pressure on the growth and beta-carotene production of Rhodotorula glutinis
- Source :
- Yeast (Chichester, England). 25(4)
- Publication Year :
- 2008
-
Abstract
- The effects of high hydrostatic pressure (HHP) on the biomass and β-carotene biosynthesis of Rhodotorula glutinis R68 were studied. After treatment with five repeated cycles at 300 MPa for 15 min, the barotolerant mutant PR68 was obtained. After 72 h of culture, the biomass of mutant PR68 was 21.6 g/l, decreased by 8.5% compared to the parental strain R68, but its β-carotene production reached 19.4 mg/l, increased by 52.8% compared to the parental strain R68. The result of restriction fragment length polymorphism (RFLP) analysis suggested that mutant strain PR68 was likely to change in nucleic acid level, and thus enhanced β-carotene production in this strain as a result of gene mutation induced by HHP treatment. Copyright © 2008 John Wiley & Sons, Ltd.
- Subjects :
- Strain (chemistry)
Mutant
Hydrostatic pressure
Rhodotorula
Bioengineering
Gene mutation
Biology
biology.organism_classification
beta Carotene
Applied Microbiology and Biotechnology
Biochemistry
Microbiology
beta-Carotene
Mutation
Genetics
Nucleic acid
Hydrostatic Pressure
Food science
Biomass
Restriction fragment length polymorphism
Chromatography, High Pressure Liquid
Polymorphism, Restriction Fragment Length
Biotechnology
Subjects
Details
- ISSN :
- 10970061
- Volume :
- 25
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Yeast (Chichester, England)
- Accession number :
- edsair.doi.dedup.....5e7425bf45ede8fd3a67ac19a3f6582f