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A direct role for murine Cdx proteins in the trunk neural crest gene regulatory network
- Source :
- Development (Cambridge, England). 143(8)
- Publication Year :
- 2015
-
Abstract
- Numerous studies in chordates and arthropods currently indicate that Cdx proteins have a major ancestral role in the organization of post-head tissues. In urochordate embryos, Cdx loss-of-function has notably been shown to impair axial elongation, neural tube (NT) closure and pigment cell development. Intriguingly, in contrast to axial elongation and NT closure, a Cdx role in neural crest (NC)-derived melanocyte/pigment cell development has not been reported in any other chordate species. To address this, we generated a new conditional pan-Cdx functional knockdown mouse model that allows circumventing Cdx functional redundancy as well as the early embryonic lethality of Cdx mutants. Through directed inhibition in the neuroectoderm, we here provide in vivo evidence that murine Cdx proteins impact melanocyte and enteric nervous system development via, at least in part, directly controlling the expression of the key early regulators of NC ontogenesis Pax3, Msx1 and FoxD3. Our work thus reveals a novel role for the Cdx proteins atop of the trunk NC-gene regulatory network in the mouse, which appears to be inherited from their ancestral orthologue.
- Subjects :
- 0301 basic medicine
Cellular differentiation
Gene regulatory network
PAX3
Biology
Models, Biological
03 medical and health sciences
Mice
medicine
Animals
Paired Box Transcription Factors
Gene Regulatory Networks
FOXD3
Molecular Biology
PAX3 Transcription Factor
Genetics
Homeodomain Proteins
MSX1 Transcription Factor
Neuroectoderm
Neural tube
Neural crest
Cell Differentiation
Forkhead Transcription Factors
Embryonic stem cell
Cell biology
Repressor Proteins
030104 developmental biology
medicine.anatomical_structure
Neural Crest
Gene Knockdown Techniques
Melanocytes
Developmental Biology
Subjects
Details
- ISSN :
- 14779129
- Volume :
- 143
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Development (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....5e86d798e524d5cabcee6747f61fd7f7