Back to Search
Start Over
Simultaneous effects of head activator on the dynamics of apical and basal regeneration in Hydra vulgaris (formerly Hydra attenuata)
- Source :
- Developmental biology. 144(1)
- Publication Year :
- 1991
-
Abstract
- Previous studies have demonstrated that head activator (HA), an 11 amino acid peptide, stimulates head-specific differentiation processes in hydra. Additionally, HA enhances the differentiation of interstitial cells into nerve cells. This study investigated the effects of exogenous synthetic HA on the dynamics of both apical and basal regeneration in a piece of tissue excised from the body column of treated animals which comprised one-eighth of the original animal. The dynamics of apical and basal regeneration were monitored using the monoclonal antibody TS19. This antibody binds to apical and basal ectodermal tissue very early in the process of regeneration, before morphological structures are evident. Labeling is ultimately localized to the tentacles of the head and a ring above the basal disc. Thus, TS19 is a useful tool for analyzing the dynamics of both apical and basal patterning processes in the same regenerate simultaneously. Quantification of TS19 positive areas on regenerates over a time course of 72 hr revealed that HA treatment accelerated and amplified the dynamics of both apical and basal TS19 labeling. The specific basal effect was novel and was demonstrated to occur in the absence of a determined head independently of new nerve cell differentiation. It is proposed that the basal effect was the result of growth factor-like activity of HA.
- Subjects :
- Activator (genetics)
medicine.drug_class
Hydra
Cellular differentiation
Antibodies, Monoclonal
Cell Biology
Anatomy
Biology
Monoclonal antibody
biology.organism_classification
Cell biology
Time course
Hydra vulgaris
medicine
biology.protein
Morphogenesis
Animals
Regeneration
Lernaean Hydra
Hydra attenuata
Antibody
Molecular Biology
Head
Developmental Biology
Subjects
Details
- ISSN :
- 00121606
- Volume :
- 144
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Developmental biology
- Accession number :
- edsair.doi.dedup.....5e8fbfe05b7a1e7bc3cd37f100fd8a52