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Effect of Spermidine Analogues on Cell Growth of Escherichia coli Polyamine Requiring Mutant MA261
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 7, p e0159494 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- The effects of spermidine analogues [norspermidine (NSPD, 33), spermidine (SPD, 34), homospermidine (HSPD, 44) and aminopropylcadaverine (APCAD, 35)] on cell growth were studied using Escherichia coli polyamine-requiring mutant MA261. Cell growth was compared at 32°C, 37°C, and 42°C. All four analogues were taken up mainly by the PotABCD spermidine-preferential uptake system. The degree of stimulation of cell growth at 32°C and 37°C was NSPD ≥ SPD ≥ HSPD > APCAD, and SPD ≥ HSPD ≥ NSPD > APCAD, respectively. However, at 42°C, it was HSPD » SPD > NSPD > APCAD. One reason for this is HSPD was taken up effectively compared with other triamines. In addition, since natural polyamines (triamines and teteraamines) interact mainly with RNA, and the structure of RNA is more flexible at higher temperatures, HSPD probably stabilized RNA more tightly at 42°C. We have thus far found that 20 kinds of protein syntheses are stimulated by polyamines at the translational level. Among them, synthesis of OppA, RpoE and StpA was more strongly stimulated by HSPD at 42°C than at 37°C. Stabilization of the initiation region of oppA and rpoE mRNA was tighter by HSPD at 42°C than 37°C determined by circular dichroism (CD). The degree of polyamine stimulation of OppA, RpoE and StpA synthesis by NSPD, SPD and APCAD was smaller than that by HSPD at 42°C. Thus, the degree of stimulation of cell growth by spermidine analogues at the different temperatures is dependent on the stimulation of protein synthesis by some components of the polyamine modulon.
- Subjects :
- 0301 basic medicine
Molecular biology
Spermidine
Mutant
lcsh:Medicine
Gene Expression
Protein Synthesis
Biochemistry
Heat Shock Response
chemistry.chemical_compound
Sigma factor
Polyamines
Protein biosynthesis
lcsh:Science
RNA structure
Cellular Stress Responses
Multidisciplinary
Messenger RNA
Escherichia coli Proteins
Norspermidine
Nucleic acids
RNA, Bacterial
Cell Processes
Research Article
Nucleic acid synthesis
Lipoproteins
Sigma Factor
Double stranded RNA
Biology
Cell Growth
03 medical and health sciences
Cadaverine
DNA-binding proteins
Genetics
Escherichia coli
Gene Regulation
Chemical synthesis
RNA synthesis
Cell Proliferation
Cell growth
lcsh:R
Biology and Life Sciences
Proteins
RNA
Cell Biology
Gene Expression Regulation, Bacterial
Regulatory Proteins
Research and analysis methods
Macromolecular structure analysis
Biosynthetic techniques
030104 developmental biology
chemistry
lcsh:Q
Mutant Proteins
Carrier Proteins
Polyamine
Transcription Factors
Molecular Chaperones
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....5ea84dd4bcb5f735aba3dea720bf5ba9