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Biofilm Formation and its Association with Antibiotic Susceptibility Pattern in Methicillin-resistant Staphylococcus aureus isolates
- Source :
- Journal of Pure and Applied Microbiology, Vol 15, Iss 4, Pp 2041-2049 (2021)
- Publication Year :
- 2021
- Publisher :
- Journal of Pure and Applied Microbiology, 2021.
-
Abstract
- Methicillin-resistant Staphylococcus aureus is a clinically significant pathogen that causes infections ranging from skin and soft tissue infections to life-threatening sepsis. Biofilm formation by MRSA is one of the crucial virulence factor. Determination of beta-lactamase and biofilm production among Staphylococcus aureus was obtained from various clinical specimens. Standard bacteriological procedures were used for isolation and identification and antibiotic sensitivity was determined using the Kirby Bauer disc diffusion method according to CLSI guidelines. The cloverleaf method, acidometric, iodometric and chromogenic methods were used to detect beta-lactamase while the microtiter plate method and Congo red agar method were used to detect biofilm production. Of the 288 MRSA strains isolated from various clinical specimens,198 (67.07%) were biofilm producers. Cloverleaf and chromogenic (nitrocefin) disc shows 100% results for beta-lactamase detection. Vancomycin was 100% sensitive followed by teicoplanin (92.36%) and linezolid (89.93%). Cloverleaf and nitrocefin disc methods were the most sensitive for detection of beta-lactamase in S. aureus and there was no significant relation between biofilm production and antibiotic sensitivity pattern of S. aureus.
- Subjects :
- medicine.drug_class
Antibiotics
Biofilm
biochemical phenomena, metabolism, and nutrition
Biology
medicine.disease_cause
Applied Microbiology and Biotechnology
Microbiology
Methicillin-resistant Staphylococcus aureus
biofilm
QR1-502
antibiotic susceptibility testing
medicine
mrsa
beta-lactamase
Biotechnology
Susceptibility pattern
Subjects
Details
- ISSN :
- 2581690X and 09737510
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Journal of Pure and Applied Microbiology
- Accession number :
- edsair.doi.dedup.....5ebac0d825cc63f599849bcae320d792
- Full Text :
- https://doi.org/10.22207/jpam.15.4.26