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Production of a codonopsis polysaccharide iron complex and evaluation of its properties
- Source :
- International journal of biological macromolecules. 162
- Publication Year :
- 2020
-
Abstract
- A water extraction and alcohol precipitation method was applied to extract polysaccharides from Codonopsis pilosula (CPP), response surface methodology was used to optimize the extraction conditions and synthesis of C. pilosula polysaccharide iron (CPPI), and the properties of CPPI were evaluated. The optimum extraction conditions for CPP were as follows: liquid-solid ratio of 29.39 mL/g, time of 1.25 h and temperature of 62.84 °C. The optimum synthesis conditions for CPPI were pH 8.9, temperature 70.30 °C and the ratio of citric acid to CPP1 of 2.95. An HPSEC-MALLS-RID system, UV spectroscopy, FT-IR spectroscopy and NMR were used for characterization of the polysaccharide. CPPI exhibited antioxidant activity in vitro and a relatively strong inhibitory effect on A2780 cells growth. After CPPI treatment, the reactive oxygen species increased, the mitochondrial membrane potential decreased, and DNA damage was observed in A2780 cells. Therefore, CPPI should be explored as a potential antioxidant and an antitumor drug in a clinical setting.
- Subjects :
- Antioxidant
medicine.medical_treatment
Iron
02 engineering and technology
Polysaccharide
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
Cell Line, Tumor
medicine
Humans
Response surface methodology
Molecular Biology
Codonopsis
030304 developmental biology
chemistry.chemical_classification
Membrane Potential, Mitochondrial
0303 health sciences
Chromatography
biology
Codonopsis pilosula
Chemistry
Extraction (chemistry)
Water extraction
General Medicine
021001 nanoscience & nanotechnology
biology.organism_classification
Antineoplastic Agents, Phytogenic
Proteoglycans
0210 nano-technology
Citric acid
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 162
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....5ec09f2d374f595f97560d9cd0fb4da2