Acaricidal activity of ethanolic extract from aerial parts of Tagetes patula L. (Asteraceae) against larvae and engorged adult females of Rhipicephalus sanguineus (Latreille, 1806)

Made available in DSpace on 2013-08-28T14:12:42Z (GMT). No. of bitstreams: 1 WOS000313219000001.pdf: 1591535 bytes, checksum: f2c5ce24fa4145acc10f5d0518b6e227 (MD5) Made available in DSpace on 2013-09-30T17:56:30Z (GMT). No. of bitstreams: 2 WOS000313219000001.pdf: 1591535 bytes, checksum: f2c5ce24fa4145acc10f5d0518b6e227 (MD5) WOS000313219000001.pdf.txt: 49405 bytes, checksum: b65b45bf3c1692970df3521019d8b782 (MD5) Previous issue date: 2012-12-17 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:54:59Z No. of bitstreams: 2 WOS000313219000001.pdf: 1591535 bytes, checksum: f2c5ce24fa4145acc10f5d0518b6e227 (MD5) WOS000313219000001.pdf.txt: 49405 bytes, checksum: b65b45bf3c1692970df3521019d8b782 (MD5) Made available in DSpace on 2014-05-20T13:55:00Z (GMT). No. of bitstreams: 2 WOS000313219000001.pdf: 1591535 bytes, checksum: f2c5ce24fa4145acc10f5d0518b6e227 (MD5) WOS000313219000001.pdf.txt: 49405 bytes, checksum: b65b45bf3c1692970df3521019d8b782 (MD5) Previous issue date: 2012-12-17 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Background: The tick Rhipicephalus sanguineus is the species with the largest worldwide distribution and is proven to be involved in the transmission of pathogens such as Babesia canis, Ehrlichia canis, Coxiella burnetii, Rickettsia ricketsii, Rickettsia conorii, among others. Studies have demonstrated acquisition of resistance to some of the active principles used in commercial formulations of acaricides. Tagetes patula (Asteraceae) is a plant with highlighted economic and commercial importance due to the production of secondary metabolites with insecticide and acaricide potential, mainly flavonoids, thiophenes and terpenes.Methods: The in vitro acaricide action of the ethanolic 70% extract from aerial parts of T. patula, obtained by percolation, was evaluated against larvae and engorged adult females of Rhipicephalus sanguineus by immersion test for 5 minutes. The chemical characterization of this extract was done by liquid chromatography coupled with mass spectrometry (LC-MS), using direct injection of sample.Results: Despite T. patula not proving lethal to adults in any of the concentrations tested, at 50.0 mg/mL oviposition rate decreased by 21.5% and eliminated 99.78% of the larvae. Also it was determined that the best results were obtained with 5 minutes of immersion. From the chromatographic analysis twelve O-glycosylated flavonoids were identified.Conclusions: This is the first report on the acaricidal activity of T. patula extract against Rh. sanguineus. If we consider the application of the product in the environment, we could completely eliminate the larval stage of development of the ixodid Rh. sanguineus. Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, UNESP, BR-14801902 Araraquara, SP, Brazil Univ Estadual Campinas, Chem Biol & Agr Pluridisciplinary Res Ctr CPQBA, UNICAMP, BR-13081970 Campinas, SP, Brazil Univ Estadual Paulista, Dept Biol, Inst Biosci, UNESP, BR-13506900 Rio Claro, SP, Brazil Univ Fed Uberlandia, Fac Vet Med, Dept Appl Immunol & Parasitol, BR-38400902 Uberlandia, MG, Brazil Univ Estadual Paulista, Coll Agr & Vet Sci, Dept Vet Pathol, UNESP, BR-14884900 Jaboticabal, SP, Brazil Univ Estadual Paulista, Dept Organ Chem, Inst Chem, UNESP, BR-14800900 Araraquara, SP, Brazil Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, UNESP, BR-14801902 Araraquara, SP, Brazil Univ Estadual Paulista, Dept Biol, Inst Biosci, UNESP, BR-13506900 Rio Claro, SP, Brazil Univ Estadual Paulista, Coll Agr & Vet Sci, Dept Vet Pathol, UNESP, BR-14884900 Jaboticabal, SP, Brazil Univ Estadual Paulista, Dept Organ Chem, Inst Chem, UNESP, BR-14800900 Araraquara, SP, Brazil