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Pervasive functional translation of noncanonical human open reading frames
- Source :
- Science. 367:1140-1146
- Publication Year :
- 2020
- Publisher :
- American Association for the Advancement of Science (AAAS), 2020.
-
Abstract
- Expanding the human proteome Using mass spectrometry, ribosome profiling, and several CRISPR-based screens, Chen et al. identified hundreds of previously uncharacterized functional micropeptides in the human genome (see the Perspective by Wei and Guo). Protein translation outside of annotated open reading frames (ORFs) in messenger RNAs and within ORFs in long noncoding RNAs is pervasive. A functional screen using CRISPR-Cas9 with single-cell transcriptomics suggested critical roles for hundreds of micropeptides. Micropeptides encoded by multiple short, upstream ORFs form stable protein complexes with the downstream canonical proteins encoded on the same messenger RNAs. Science , this issue p. 1140 ; see also p. 1074
- Subjects :
- 0303 health sciences
Messenger RNA
Multidisciplinary
RNA
Translation (biology)
Computational biology
Biology
Transcriptome
Open Reading Frames
03 medical and health sciences
Open reading frame
0302 clinical medicine
Protein Biosynthesis
030220 oncology & carcinogenesis
Operon
Humans
CRISPR
RNA, Messenger
Ribosome profiling
CRISPR-Cas Systems
Peptides
Ribosomes
030304 developmental biology
Genetic screen
Subjects
Details
- ISSN :
- 10959203 and 00368075
- Volume :
- 367
- Database :
- OpenAIRE
- Journal :
- Science
- Accession number :
- edsair.doi.dedup.....5edf0fdf6d44d83ddd06461f4d7e93e8