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Deposition of the spermatozoon in the human oocyte at ICSI: impact on oocyte survival, fertilization and blastocyst formation

Authors :
G. Verheyen
A. De Vos
P. Haentjens
M. Abraham
N. Franceus
H. Tournaye
H. Van de Velde
Surgical clinical sciences
Internal Medicine Specializations
Biology of the Testis
Centre for Reproductive Medicine - Gynaecology
Basic (bio-) Medical Sciences
Reproduction and Genetics
Reproductive immunology and implantation
Source :
Journal of Assisted Reproduction and Genetics. 32:865-871
Publication Year :
2015
Publisher :
Springer Science and Business Media LLC, 2015.

Abstract

PURPOSE: To evaluate whether the deposition of the spermatozoon in the human oocyte at ICSI has any effect on oocyte survival, fertilization, blastocyst development and quality. METHODS: In a prospective study, including 78 ICSI cycles, sibling oocytes were injected with "no intention" (group A, standard ICSI, n = 393) or "intention" to deposit the spermatozoon under the cortex (group B, n = 354). Outcome parameters were oocyte survival and fertilization, as well as blastocyst formation and quality. RESULTS: Depositing the sperm under the cortex of the oocyte was not always successful for its final position, therefore, group B was divided into three subgroups: B1 successful deposition (119 oocytes, 33.6 % of oocytes in group B); B2 initially successful but spermatozoon spontaneously relocated after 2 min (136 oocytes, 38.4 %); and B3 unsuccessful deposition (99 oocytes, 28.0 %). Group A and B were compared on an intention-to-treat basis. Additionally, A, B1, B2 and B3 were also compared. Theoocyte survival and fertilization, blastocyst and top-quality blastocyst developmental rates were not significantly different. CONCLUSIONS: The procedure of depositing the spermatozoon intentionally under the oocyte cortex demanded high technical skills. Successful positioning was only obtained in 34 % of the attempts. We obtained no evidence of improved oocyte survival and fertilization, blastocyst formation and quality when the spermatozoon was permanently positioned under the oocyte cortex. Taken together, depositing the spermatozoon under the oocyte cortex is not recommended for routine ICSI application.

Details

ISSN :
15737330 and 10580468
Volume :
32
Database :
OpenAIRE
Journal :
Journal of Assisted Reproduction and Genetics
Accession number :
edsair.doi.dedup.....5ef35c92436648a4e43d05cbbd2c3ba9
Full Text :
https://doi.org/10.1007/s10815-015-0482-6