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Laser engraved microapillary pump paper-based microfluidic device for colorimetric and electrochemical detection of salivary thiocyanate
- Source :
- Mikrochimica acta. 188(4)
- Publication Year :
- 2020
-
Abstract
- A microcapillary grooved paper-based analytical device capable of dual-mode sensing (colorimetric and electrochemical detection) was demonstrated for analysis of viscous samples (e.g., human saliva). Herein, a hollow capillary channel was constructed via laser engraved micropatterning functions as a micropump to facilitate viscous fluidic transport, which would otherwise impede analysis on paper devices. Using salivary thiocyanate as a model analyte, the proposed device was found to exhibit a promising sensing ability on paper devices without the need for sample pretreatment or bulky instrumentation, as normally required in conventional methods used for saliva analysis. An extensive linear dynamic range covering detection of salivary thiocyanate for both high and trace level regimes (5 orders of magnitude working range) was collectively achieved using the dual-sensing modes. Under optimal conditions, the limit of detection was 6 μmol L−1 with a RSD of less than 5%. An excellent stability for the μpumpPAD was also observed for over 30 days. Real sample analysis using the proposed device was found to be in line with the standard chromatographic method. Benefitting from simple fabrication and operation, portability, disposability, low sample volume (20 μL), and low cost (< 1 USD), the μpumpPAD is an exceptional alternative tool for the detection of various biomarkers in saliva specimens.
- Subjects :
- Paper
Analyte
Materials science
Indoles
Microfluidics
Micropump
Analytical Chemistry
chemistry.chemical_compound
Limit of Detection
Lab-On-A-Chip Devices
Organometallic Compounds
Humans
Fluidics
Saliva
Electrodes
Detection limit
Smokers
Thiocyanate
business.industry
Dynamic range
Lasers
Electrochemical Techniques
Non-Smokers
Microfluidic Analytical Techniques
chemistry
Optoelectronics
Colorimetry
Graphite
business
Thiocyanates
Micropatterning
Subjects
Details
- ISSN :
- 14365073
- Volume :
- 188
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Mikrochimica acta
- Accession number :
- edsair.doi.dedup.....5ef9d39884c6dbd99979337aea225b31