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Noninvasive Optical Assessment of Implanted Tissue-Engineered Construct Success In Situ
- Source :
- Tissue Eng Part C Methods
- Publication Year :
- 2021
- Publisher :
- Mary Ann Liebert Inc, 2021.
-
Abstract
- Quantitative diffuse reflectance spectroscopy (DRS) was developed for label-free, noninvasive, and real-time assessment of implanted tissue-engineered devices manufactured from primary human oral keratinocytes (six batches in two 5-patient cohorts). Constructs were implanted in a murine model for 1 and 3 weeks. DRS evaluated construct success in situ using optical absorption (hemoglobin concentration and oxygenation, attributed to revascularization) and optical scattering (attributed to cellular density and layer thickness). Destructive pre- and postimplantation histology distinguished experimental control from stressed constructs, whereas noninvasive preimplantation measures of keratinocyte glucose consumption and residual glucose in spent culture media did not. In constructs implanted for 1 week, DRS distinguished control due to stressed and compromised from healthy constructs. In constructs implanted for 3 weeks, DRS identified constructs with higher postimplantation success. These results suggest that quantitative DRS is a promising, clinically compatible technology for rapid, noninvasive, and localized tissue assessment to characterize tissue-engineered construct success in vivo. IMPACT STATEMENT: Despite the recent advance in tissue engineering and regenerative medicine, there is still a lack of nondestructive tools to longitudinally monitor the implanted tissue-engineered devices. In this study, we demonstrate the potential of quantitative diffuse reflectance spectroscopy as a clinically viable technique for noninvasive, label-free, and rapid characterization of graft success in situ.
- Subjects :
- Keratinocytes
In situ
Experimental control
0206 medical engineering
Biomedical Engineering
Medicine (miscellaneous)
Cell Count
Bioengineering
02 engineering and technology
Regenerative medicine
Mice
03 medical and health sciences
Tissue engineering
In vivo
Animals
Humans
Medicine
030304 developmental biology
0303 health sciences
Tissue engineered
Tissue Engineering
Tissue Scaffolds
business.industry
020601 biomedical engineering
Layer thickness
Methods Articles
Murine model
business
Biomedical engineering
Subjects
Details
- ISSN :
- 19373392 and 19373384
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Tissue Engineering Part C: Methods
- Accession number :
- edsair.doi.dedup.....5f1926d6a5576cf73c5dfc5ac9d995d1